Evidence map›Paper›PMID 29275446›Full record

ArticleDigestive diseases and sciences2018

Distinct Expression of Phenotypic Markers in Placodes- and Neural Crest-Derived Afferent Neurons Innervating the Rat Stomach.

Alzbeta Trancikova, Eva Kovacova, Fei Ru, Kristian Varga, Mariana Brozmanova, Milos Tatar, Marian Kollarik

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Article in Digestive diseases and sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Alzbeta TrancikovaComenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Malá Hora 4C, 036 01, Martin, Slovakia.
Eva KovacovaComenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Malá Hora 4C, 036 01, Martin, Slovakia.
Fei RuDepartment of Medicine, The Johns Hopkins University School of Medicine, Johns Hopkins Asthma Center, RM 1A.2, 5501 Hopkins Bayview Circle, Baltimore, MD, 21224, USA.
Kristian VargaComenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Malá Hora 4C, 036 01, Martin, Slovakia.
Mariana BrozmanovaComenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Malá Hora 4C, 036 01, Martin, Slovakia.
Milos TatarComenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Malá Hora 4C, 036 01, Martin, Slovakia.
Marian KollarikDepartment of Medicine, The Johns Hopkins University School of Medicine, Johns Hopkins Asthma Center, RM 1A.2, 5501 Hopkins Bayview Circle, Baltimore, MD, 21224, USA. kollarik@jhmi.edu.ORCID 0000-0002-9659-5007
Comenius University Bratislava · SKJohns Hopkins University · US

Funding

Agentúra na Podporu Výskumu a Vývoja APVV-15-0163NIH HHS DK110366
6 · The paper itself

Abstract

backgroundVisceral pain is initiated by activation of primary afferent neurons among which the capsaicin-sensitive (TRPV1-positive) neurons play an important role. The stomach is a common source of visceral pain. Similar to other organs, the stomach receives dual spinal and vagal afferent innervation. Developmentally, spinal dorsal root ganglia (DRG) and vagal jugular neurons originate from embryonic neural crest and vagal nodose neurons originate from placodes. In thoracic organs the neural crest- and placodes-derived TRPV1-positive neurons have distinct phenotypes differing in activation profile, neurotrophic regulation and reflex responses. It is unknown to whether such distinction exists in the stomach.

aimsWe hypothesized that gastric neural crest- and placodes-derived TRPV1-positive neurons express phenotypic markers indicative of placodes and neural crest phenotypes.

methodsGastric DRG and vagal neurons were retrogradely traced by DiI injected into the rat stomach wall. Single-cell RT-PCR was performed on traced gastric neurons.

resultsRetrograde tracing demonstrated that vagal gastric neurons locate exclusively into the nodose portion of the rat jugular/petrosal/nodose complex. Gastric DRG TRPV1-positive neurons preferentially expressed markers PPT-A, TrkA and GFRα

conclusionsThere are phenotypic distinctions between the neural crest-derived DRG and placodes-derived vagal nodose TRPV1-positive neurons innervating the rat stomach that are similar to those described in thoracic organs.

Indexed as

AnimalsBiomarkersCarbocyaninesGene Expression RegulationMaleNeural CrestNeurons, AfferentRatsRats, Sprague-DawleyStomachTRPV Cation ChannelsVagus Nerve3,3'-dioctadecylindocarbocyanineBiomarkersCarbocyaninesTrpv1 protein, ratTRPV Cation ChannelsAfferent neuronsNeural crestPlacodesStomachTRPV1Vagus nerve

Identifiers

PMID29275446
OpenAlexW2777108274

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.