ArticleScientific reports2017
The stringent response promotes biofilm dispersal in Pseudomonas putida.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Plant Extracts Target Biofilms to Alleviate Nutritional and Metabolic Disorders in Animals.Veterinary sciences · 2026Review
- The opposite effects of stringent response on phage infection ofmicroLife · 2026Article
- Competition between Pseudomonas species constrains ecological diversification in polymicrobial biofilms.NPJ biofilms and microbiomes · 2025Article
- Article
- Membrane filtration reduces nutrient availability and invasion potential in drinking water systems, without affecting mature biofilms.Frontiers in microbiology · 2025Article
- Impact of Nutrient Starvation on Biofilm Formation inAntibiotics (Basel, Switzerland) · 2024Article
- Dispersal of a dominant competitor can drive multispecies coexistence in biofilms.Current biology : CB · 2024Article
- Intestinal biofilms: pathophysiological relevance, host defense, and therapeutic opportunities.Clinical microbiology reviews · 2024Review
- Physiology, fast and slow: bacterial response to variable resource stoichiometry and dilution rate.mSystems · 2024Article
- Binding of GTP to BifA is required for the production of Pel-dependent biofilms inJournal of bacteriology · 2024Article
- Bacterial respiratory inhibition triggers dispersal ofApplied and environmental microbiology · 2023Article
- Tale of Twin Bifunctional Second Messenger (p)ppGpp Synthetases and Their Function in Mycobacteria.ACS omega · 2023Review
- Teg58, a small regulatory RNA, is involved in regulating arginine biosynthesis and biofilm formation in Staphylococcus aureus.Scientific reports · 2022Article
- Article
- Article
- Nutrient Sensing and Biofilm Modulation: The Example of L-arginine inInternational journal of molecular sciences · 2022Review
- Tryptone in Growth Media EnhancesMicroorganisms · 2022Article
- Two (p)ppGpp Synthetase Genes,Frontiers in microbiology · 2022Article
- Dynamic Metabolic Response to (p)ppGpp Accumulation inFrontiers in microbiology · 2022Article
- Collective behavior and virulence arsenal of the fish pathogenFrontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm dispersal is a genetically programmed response enabling bacterial cells to exit the biofilm in response to particular physiological or environmental conditions. In Pseudomonas putida biofilms, nutrient starvation triggers c-di-GMP hydrolysis by phosphodiesterase BifA, releasing inhibition of protease LapG by the c-di-GMP effector protein LapD, and resulting in proteolysis of the adhesin LapA and the subsequent release of biofilm cells. Here we demonstrate that the stringent response, a ubiquitous bacterial stress response, is accountable for relaying the nutrient stress signal to the biofilm dispersal machinery. Mutants lacking elements of the stringent response - (p)ppGpp sythetases [RelA and SpoT] and/or DksA - were defective in biofilm dispersal. Ectopic (p)ppGpp synthesis restored biofilm dispersal in a ∆relA ∆spoT mutant. In vivo gene expression analysis showed that (p)ppGpp positively regulates transcription of bifA, and negatively regulates transcription of lapA and the lapBC, and lapE operons, encoding a LapA-specific secretion system. Further in vivo and in vitro characterization revealed that the PbifA promoter is dependent on the flagellar σ factor FliA, and positively regulated by ppGpp and DksA. Our results indicate that the stringent response stimulates biofilm dispersal under nutrient limitation by coordinately promoting LapA proteolysis and preventing de novo LapA synthesis and secretion.
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