ArticleScientific reports2017
LUCS (Light-Up Cell System), a universal high throughput assay for homeostasis evaluation in live cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
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- Bioactivity of Grape Pomace Extract and Sodium Selenite, Key Components of the OenoGrape Advanced Complex, on Target Human Cells: Intracellular ROS Scavenging and Nrf2/ARE Induction Following In Vitro Intestinal Absorption.Antioxidants (Basel, Switzerland) · 2024Article
- A New Role for Yeast Cells in Health and Nutrition: Antioxidant Power Assessment.International journal of molecular sciences · 2023Article
- Cellular Antioxidant Effect of an Aronia Extract and Its Polyphenolic Fractions Enriched in Proanthocyanidins, Phenolic Acids, and Anthocyanins.Antioxidants (Basel, Switzerland) · 2022Article
- Metabolomics-Based Profiling, Antioxidant Power, and Uropathogenic Bacterial Anti-Adhesion Activity of SP4Antioxidants (Basel, Switzerland) · 2022Article
- Cell-Based Antioxidant Properties and Synergistic Effects of Natural Plant and Algal Extracts Pre and Post Intestinal Barrier Transport.Antioxidants (Basel, Switzerland) · 2022Article
- Live Cell Assays for the Assessment of Antioxidant Activities of Plant Extracts.Antioxidants (Basel, Switzerland) · 2021Review
- Association of NQO2 With UDP-Glucuronosyltransferases Reduces Menadione Toxicity in Neuroblastoma Cells.Frontiers in pharmacology · 2021Article
- A short review on cell-based biosensing: challenges and breakthroughs in biomedical analysis.Journal of biomedical research · 2020Article
- The Extent of Intracellular Accumulation of Bilirubin Determines Its Anti- or Pro-Oxidant Effect.International journal of molecular sciences · 2020Article
- AOP1, a New Live Cell Assay for the Direct and Quantitative Measure of Intracellular Antioxidant Effects.Antioxidants (Basel, Switzerland) · 2020Article
- Cellular sensing platform with enhanced sensitivity based on optogenetic modulation of cell homeostasis.Biosensors & bioelectronics · 2020Article
- Modulation of Cellular Reactivity for Enhanced Cell-Based Biosensing.Analytical chemistry · 2020Article
- Use of LUCS (Light-Up Cell System) as an alternative live cell method to predict human acute oral toxicity.Toxicology reports · 2020Article
- Terminalia albida treatment improves survival in experimental cerebral malaria through reactive oxygen species scavenging and anti-inflammatory properties.Malaria journal · 2019Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Observations of fluorescent cyanine dye behavior under illumination at 500 nm lead to a novel concept in cell biology allowing the development of a new live cell assay called LUCS, for Light-Up Cell System, measuring homeostasis in live cells. Optimization of the LUCS process resulted in a standardized, straightforward and high throughput assay with applications in toxicity assessment. The mechanisms of the LUCS process were investigated. Electron Paramagnetic Resonance experiments showed that the singlet oxygen and hydroxyl radical are involved downstream of the light effect, presumably leading to deleterious oxidative stress that massively opens access of the dye to its intracellular target. Reversible modulation of LUCS by both verapamil and proton availability indicated that plasma membrane proton/cation antiporters, possibly of the MATE drug efflux transport family, are involved. A mechanistic model is presented. Our data show that intracellular oxidation can be controlled by tuning light energy, opening applications in regulatory purposes, anti-oxidant research, chemotherapy efficacy and dynamic phototherapy strategies.
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