Evidence map›Paper›PMID 29273626›Full record

ArticleGenome research2018

MinION-based long-read sequencing and assembly extends the

John R Tyson, Nigel J O'Neil, Miten Jain, Hugh E Olsen, Philip Hieter, Terrance P Snutch

Open access · bronzeAbstract read
In one paragraph

Article in Genome research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 151 citations in OpenAlex.

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  18. Hybrid Genome Assembly of Short and Long Reads in Galaxy.Methods in molecular biology (Clifton, N.J.) · 2023
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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

John R Tyson *Michael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
Nigel J O'Neil *Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
Miten Jain *UC Santa Cruz Genomics Institute and Department of Biomolecular Engineering, University of California, Santa Cruz, California 95064, USA.
Hugh E OlsenUC Santa Cruz Genomics Institute and Department of Biomolecular Engineering, University of California, Santa Cruz, California 95064, USA.
Philip HieterMichael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
Terrance P SnutchMichael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
University of British Columbia · CAUniversity of California, Santa Cruz · US

Funding

Training SubprojectU54HG007990 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI HAUSSLER, DAVID H, PATTERSON, DAVID · 2014 to 2018
$14.8M
Optimization of Processive Enzymes for DNA Sequencing using NanoporesR01HG006321 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI AKESON, MARK A · 2011 to 2013
$3.6M
Optimization of Nanopore Genomic DNA SequencingR01HG007827 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI AKESON, MARK A · 2014 to 2016
$2.2M
NHGRI NIH HHS R01 HG006321NHGRI NIH HHS R01 HG007827NHGRI NIH HHS U54 HG007990
6 · The paper itself

Abstract

Advances in long-read single molecule sequencing have opened new possibilities for 'benchtop' whole-genome sequencing. The Oxford Nanopore Technologies MinION is a portable device that uses nanopore technology that can directly sequence DNA molecules. MinION single molecule long sequence reads are well suited for de novo assembly of complex genomes as they facilitate the construction of highly contiguous physical genome maps obviating the need for labor-intensive physical genome mapping. Long sequence reads can also be used to delineate complex chromosomal rearrangements, such as those that occur in tumor cells, that can confound analysis using short reads. Here, we assessed MinION long-read-derived sequences for feasibility concerning: (1) the de novo assembly of a large complex genome, and (2) the elucidation of complex rearrangements. The genomes of two

Indexed as

Molecular Sequence AnnotationAnimalsCaenorhabditis elegansChromosome MappingGene RearrangementGenomeHigh-Throughput Nucleotide SequencingRepetitive Sequences, Nucleic Acid

Identifiers

PMID29273626
PMCPMC5793790
OpenAlexW2781129742

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.