Evidence map›Paper›PMID 29273248›Full record

ArticleToxicon : official journal of the International Society on Toxinology2018

Substrate cleavage and duration of action of botulinum neurotoxin type FA ("H, HA").

Sabine Pellett, William H Tepp, Guangyun Lin, Eric A Johnson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicon : official journal of the International Society on Toxinology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Frontiers in microbiology · 2022
    Review
  4. Article
  5. Review
  6. Novel Native and Engineered Botulinum Neurotoxins.Handbook of experimental pharmacology · 2021
    Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Sabine PellettDepartment of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison, WI 53706, USA. Electronic address: Sabine.pellett@wisc.edu.
William H TeppDepartment of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison, WI 53706, USA. Electronic address: whtepp@wisc.edu.
Guangyun LinDepartment of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison, WI 53706, USA. Electronic address: glin2@wisc.edu.
Eric A JohnsonDepartment of Bacteriology, University of Wisconsin, 1550 Linden Dr., Madison, WI 53706, USA. Electronic address: eric.johnson@wisc.edu.
University of Wisconsin–Madison · US

Funding

Mechanisms of Bacterial Toxin ActionR01AI030162 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI BARBIERI, JOSEPH T · 1990 to 2019
$6.7M
Vaccines Against BotulismR01AI118389 · NIAID · MEDICAL COLLEGE OF WISCONSIN · PI BARBIERI, JOSEPH T, PELLETT, SABINE · 2015 to 2024
$6.1M
Characterization of Botulinum Neurotoxin A SubtypesR01AI095274 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI JOHNSON, ERIC A. · 2012 to 2016
$2.0M
Characterization of botulinum neurotoxin A subtypesR56AI095274 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BARBIERI, JOSEPH T, JOHNSON, ERIC A. · 2017 to 2017
$564k
NIAID NIH HHS R01 AI030162NIAID NIH HHS R01 AI095274NIAID NIH HHS R01 AI118389NIAID NIH HHS R56 AI095274
6 · The paper itself

Abstract

Botulinum neurotoxin (BoNT) type FA is the only known naturally occurring chimeric BoNT of domains of BoNT/A and BoNT/F. BoNT/FA consists of an F5-like light chain (LC), a unique heavy chain (HC) translocation domain, and a HC receptor binding domain similar to BoNT/A1. Previous analyses of purified BoNT/FA have indicated a 5-10-fold greater potency in cultured human or rat neurons as compared to BoNT/A1 and a 400-500-fold greater potency compared to BoNT/B1. However, in vivo potency in mice was about 5-fold lower than BoNT/A1 or/B1. In this report, species specificity was examined by cell-based assays using primary neurons from mice and examining VAMP1 and 2 cleavage. The data indicated similar potency of BoNT/FA in primary mouse spinal cord neurons as previously observed in primary rat and human induced pluripotent stem cell (hiPSC) derived neuronal cell models, and equal enzymatic cleavage of mouse VAMP1 and 2 isoforms. Since the duration of action of BoNTs is due to continuous enzymatic activity of the LC in the neuronal cytosol, BoNT/FA was expected to have a short duration of action due to its F-type LC. In this report the duration of action of BoNT/FA was compared to that of BoNT/F1,/F5, and/B1 in both hiPSC derived neurons and in the in vivo mouse model. The data indicate a duration of action of BoNT/FA similar to BoNT/B1, while BoNT/F5 had a short duration of action similar to BoNT/F1.

Indexed as

AnimalsBotulinum ToxinsCells, CulturedMiceMotor ActivityNeuronsParalysisSpinal CordVesicle-Associated Membrane Protein 1Vesicle-Associated Membrane Protein 2Botulinum ToxinsVesicle-Associated Membrane Protein 1vesicle-associated membrane protein 1, mouseVesicle-Associated Membrane Protein 2vesicle-associated membrane protein 2, mouseBotulinum neurotoxinBotulinum neurotoxin FABotulinum neurotoxin HDurationRecoveryVAMP

Identifiers

PMID29273248
PMCPMC5911199
OpenAlexW2778849518

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.