ArticleBMC genomics2017
A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Exosome-Mediated miRNA Delivery Restores Early Differentiation and Survival Programs in DGCR8-Deficient Mouse Embryonic Stem Cells.International journal of molecular sciences · 2026Article
- Benchmarking transcriptome deconvolution methods for estimating tissue- and cell-type-specific extracellular vesicle abundances.Journal of extracellular vesicles · 2024Article
- Oxidative stress-induced changes in the transcriptomic profile of extracellular vesicles.Journal of extracellular biology · 2024Article
- Molecular characterization of colorectal adenoma and colorectal cancer via integrated genomic transcriptomic analysis.Frontiers in oncology · 2023Article
- Plasma Exosomes Transfer miR-885-3p Targeting the AKT/NFκB Signaling Pathway to Improve the Sensitivity of Intravenous Glucocorticoid Therapy Against Graves Ophthalmopathy.Frontiers in immunology · 2022Article
- Prognostic and immunological role of cuproptosis-related protein FDX1 in pan-cancer.Frontiers in genetics · 2022Article
- Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis.iScience · 2021Article
- Adipocyte-derived extracellular vesicles in health and diseases: Nano-packages with vast biological properties.FASEB bioAdvances · 2021Review
- CD5L as an Extracellular Vesicle-Derived Biomarker for Liquid Biopsy of Lung Cancer.Diagnostics (Basel, Switzerland) · 2021Article
- Technological Approaches in the Analysis of Extracellular Vesicle Nucleotide Sequences.Frontiers in bioengineering and biotechnology · 2021Review
- Application of Sparse Representation in Bioinformatics.Frontiers in genetics · 2021Review
- Genomic, proteomic, and systems biology approaches in biomarker discovery for multiple sclerosis.Cellular immunology · 2020Review
- The S100B Protein and Partners in Adipocyte Response to Cold Stress and Adaptive Thermogenesis: Facts, Hypotheses, and Perspectives.Biomolecules · 2020Review
- Effects of different separation methods on the physical and functional properties of extracellular vesicles.PloS one · 2020Article
- Review
- Roles and Regulation of Extracellular Vesicles in Cardiovascular Mineral Metabolism.Frontiers in cardiovascular medicine · 2018Review
Corrections and comments
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Authors and funding
21 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExosomes and other extracellular vesicles (EVs) have emerged as an important mechanism of cell-to-cell communication. However, previous studies either did not fully resolve what genetic materials were shuttled by exosomes or only focused on a specific set of miRNAs and mRNAs. A more systematic method is required to identify the genetic materials that are potentially transferred during cell-to-cell communication through EVs in an unbiased manner.
resultsIn this work, we present a novel next generation of sequencing (NGS) based approach to identify EV mediated mRNA exchanges between co-cultured adipocyte and macrophage cells. We performed molecular and genomic profiling and jointly considered data from RNA sequencing (RNA-seq) and genotyping to track the "sequence varying mRNAs" transferred between cells. We identified 8 mRNAs being transferred from macrophages to adipocytes and 21 mRNAs being transferred in the opposite direction. These mRNAs represented biological functions including extracellular matrix, cell adhesion, glycoprotein, and signal peptides.
conclusionsOur study sheds new light on EV mediated RNA communications between adipocyte and macrophage cells, which may play a significant role in developing insulin resistance in diabetic patients. This work establishes a new method that is applicable to examining genetic material exchanges in many cellular systems and has the potential to be extended to in vivo studies as well.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.