Evidence map›Paper›PMID 29272914›Full record

ArticleBioconjugate chemistry2018

Synthesis and Characterization of a Magnetically Active

Megan E Dempsey, Hetal D Marble, Tun-Li Shen, Nicolas L Fawzi, Eric M Darling

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioconjugate chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Advances in RNA Labeling with Trifluoromethyl Groups.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023
    Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Megan E DempseyCenter for Biomedical Engineering, ‡Department of Molecular Pharmacology, Physiology, and Biotechnology, §Department of Chemistry, ∥School of Engineering, and ⊥Department of Orthopaedics, Brown University , Providence, Rhode Island 02912, United States.ORCID 0000-0003-0207-4989
Hetal D MarbleCenter for Biomedical Engineering, ‡Department of Molecular Pharmacology, Physiology, and Biotechnology, §Department of Chemistry, ∥School of Engineering, and ⊥Department of Orthopaedics, Brown University , Providence, Rhode Island 02912, United States.
Tun-Li ShenCenter for Biomedical Engineering, ‡Department of Molecular Pharmacology, Physiology, and Biotechnology, §Department of Chemistry, ∥School of Engineering, and ⊥Department of Orthopaedics, Brown University , Providence, Rhode Island 02912, United States.
Nicolas L FawziCenter for Biomedical Engineering, ‡Department of Molecular Pharmacology, Physiology, and Biotechnology, §Department of Chemistry, ∥School of Engineering, and ⊥Department of Orthopaedics, Brown University , Providence, Rhode Island 02912, United States.ORCID 0000-0001-5483-0577
Eric M DarlingCenter for Biomedical Engineering, ‡Department of Molecular Pharmacology, Physiology, and Biotechnology, §Department of Chemistry, ∥School of Engineering, and ⊥Department of Orthopaedics, Brown University , Providence, Rhode Island 02912, United States.ORCID 0000-0002-3932-8623
Brown University · US

Funding

High-yield, lineage-specific enrichment of living mesenchymal stem cellsR01AR063642 · NIAMS · BROWN UNIVERSITY · PI DARLING, ERIC M · 2013 to 2018
$1.8M
NIAMS NIH HHS R01 AR063642
6 · The paper itself

Abstract

Gene expression is used extensively to describe cellular characteristics and behaviors; however, most methods of assessing gene expression are unsuitable for living samples, requiring destructive processes such as fixation or lysis. Recently, molecular beacons have become a viable tool for live-cell imaging of mRNA molecules in situ. Historically, beacon-mediated imaging has been limited to fluorescence-based approaches. We propose the design and synthesis of a novel molecular beacon for magnetic resonance detection of any desired target nucleotide sequence. The biologically compatible synthesis incorporates commonly used bioconjugation reactions in aqueous conditions and is accessible for laboratories without extensive synthesis capabilities. The resulting beacon uses fluorine (

Indexed as

Fluorescent DyesFluorineGene ExpressionMagnetic Resonance SpectroscopyNucleic Acid HybridizationOligonucleotide ProbesRNA, MessengerFluorescent DyesFluorineOligonucleotide ProbesRNA, Messenger

Identifiers

PMID29272914
PMCPMC5821531
OpenAlexW2776130339

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.