ReviewPlant biotechnology journal2018
A guide to the contained use of plant virus infectious clones.
Review in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- Local citrus sudden death-associated virus infection inThe Journal of general virology · 2026Article
- Viral vectors for antimicrobial peptide expression: a new path for crop protection.Frontiers in microbiology · 2026Review
- Development of a Reverse Genetics System for Rice Tiller Inhibition Virus.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Construction and Segmental Reconstitution of Full-Length Infectious Clones of Milk Vetch Dwarf Virus.Viruses · 2025Article
- Article
- Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus.Virus research · 2024Article
- Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon.The plant pathology journal · 2024Article
- Plant-made pharmaceuticals.Plant biotechnology (Tokyo, Japan) · 2024Article
- Development of Stable Infectious cDNA Clones of Tomato Black Ring Virus Tagged with Green Fluorescent Protein.Viruses · 2024Article
- Assembly of plant virus agroinfectious clones using biological material or DNA synthesis.STAR protocols · 2022Article
- Viruses of Yams (Viruses · 2022Review
- VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants.Stress biology · 2022Review
- Alcohol-perturbed self-assembly of the tobacco mosaic virus coat protein.Beilstein journal of nanotechnology · 2022Article
- A cassava common mosaic virus vector for virus-induced gene silencing in cassava.Plant methods · 2021Article
- Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications.Biology · 2021Review
- Review
- Synthesis of Full-Length cDNA Infectious Clones ofViruses · 2020Article
- Utilization of infectious clones to visualize Cassava brown streak virus replication in planta and gain insights into symptom development.Virus genes · 2019Article
- Cassava brown streak virus Ham1 protein hydrolyses mutagenic nucleotides and is a necrosis determinant.Molecular plant pathology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Plant virus infectious clones are important tools with wide-ranging applications in different areas of biology and medicine. Their uses in plant pathology include the study of plant-virus interactions, and screening of germplasm as part of prebreeding programmes for virus resistance. They can also be modified to induce transient plant gene silencing (Virus Induced Gene Silencing - VIGS) and as expression vectors for plant or exogenous proteins, with applications in both plant pathology and more generally for the study of plant gene function. Plant viruses are also increasingly being investigated as expression vectors for in planta production of pharmaceutical products, known as molecular farming. However, plant virus infectious clones may pose a risk to the environment due to their ability to reconstitute fully functional, transmissible viruses. These risks arise from both their inherent pathogenicity and the effect of any introduced genetic modifications. Effective containment measures are therefore required. There has been no single comprehensive review of the biosafety considerations for the contained use of genetically modified plant viruses, despite their increasing importance across many biological fields. This review therefore explores the biosafety considerations for working with genetically modified plant viruses in contained environments, with focus on plant growth facilities. It includes regulatory frameworks, risk assessment, assignment of biosafety levels, facility features and working practices. The review is based on international guidance together with information provided by plant virus researchers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.