Evidence map›Paper›PMID 29270750›Full record

ArticleJournal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban2017

RNAi-mediated human Nestin silence inhibits proliferation and migration of malignant melanoma cells by G

Xu-Hui Yang, Tian Xia, Jie Zhang, Shao-Fen Yang, Hui-Xia Tang, Ting Tang, Zhi-Cheng Huang, Yue-Si Zhong, Feng He, Andy Peng Xiang

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Xu-Hui YangAssisted Reproductive Center, Guangdong Women and Children's Hospital, Guangzhou, 510010, China.
Tian XiaCenter for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou, 510080, China.
Jie ZhangAssisted Reproductive Center, Guangdong Women and Children's Hospital, Guangzhou, 510010, China.
Shao-Fen YangAssisted Reproductive Center, Guangdong Women and Children's Hospital, Guangzhou, 510010, China.
Hui-Xia TangAssisted Reproductive Center, Guangdong Women and Children's Hospital, Guangzhou, 510010, China.
Ting TangAssisted Reproductive Center, Guangdong Women and Children's Hospital, Guangzhou, 510010, China.
Zhi-Cheng HuangAssisted Reproductive Center, Guangdong Women and Children's Hospital, Guangzhou, 510010, China.
Yue-Si ZhongDepartment of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
Feng HeSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China. hefeng@mail.sysu.edu.cn.
Andy Peng XiangCenter for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou, 510080, China. xiangp@mail.sysu.edu.cn.
Guangdong Province Women and Children Hospital · CNSun Yat-sen University · CNChildren's Hospital of Zhejiang University · CNThird Affiliated Hospital of Sun Yat-sen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human Nestin (hNestin) has been found to express in melanoma, and its expression is positively correlated with the advanced stage of melanoma. However, the precise role of hNestin in the development of melanoma has not been fully understood. The present study aimed to explore the role of hNestin in the proliferation and invasion of melanoma cells. The lentivirus vector carrying a short hairpin RNAs (shRNAs) targeting hNestin (hNestin-shRNA-LV) was stably infected into human melanoma cells UACC903, which expressed high levels of hNestin. The effects of hNestin knockdown on the proliferation, apoptosis, migration of melanoma cells and the related signaling pathways were investigated by immunofluorence, Western blotting and reverse transcription polymerase chain reaction (RT-PCR), respectively. The results showed that hNestin was expressed in most melanoma specimens and the melanoma cells studied. Knockdown of hNestin expression significantly inhibited the proliferation of melanoma cells, blocked the formation of cell colony, arrested cell cycle at G

Indexed as

Gene Expression Regulation, NeoplasticAntigens, CDApoptosisbeta CateninCadherinsCell Line, TumorCell MovementCell ProliferationEpithelial-Mesenchymal TransitionG1 Phase Cell Cycle CheckpointsGenetic VectorsGlycogen Synthase Kinase 3 betaHumansLentivirusMelanomaNestinAntigens, CDbeta CateninCadherinsCDH2 protein, humanCTNNB1 protein, humanGlycogen Synthase Kinase 3 betaGSK3B protein, humanNES protein, humanNestinProto-Oncogene Proteins c-aktRetinoblastoma ProteinRNA, Small InterferinghNestinmelanoma cellmigrationproliferationRNA interference

Identifiers

PMID29270750
OpenAlexW2783581454

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.