ArticleFungal biology and biotechnology2017
ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in
Article in Fungal biology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 84 citations in OpenAlex.
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- A genetic tool to express long fungal biosynthetic genes.Fungal biology and biotechnology · 2023Article
- Metabolomic profiles of the liquid state fermentation in co-culture ofFrontiers in microbiology · 2023Article
- Modular Inducible Multigene Expression System for Filamentous Fungi.Microbiology spectrum · 2022Article
- Blue Light-Dependent Pre-mRNA Splicing Controls Pigment Biosynthesis in the MushroomMicrobiology spectrum · 2022Article
- Unprecedented Mushroom Polyketide Synthases Produce the Universal Anthraquinone Precursor.Angewandte Chemie (International ed. in English) · 2022Article
- Longitudinal multimodal imaging-compatible mouse model of triazole-sensitive and -resistant invasive pulmonary aspergillosis.Disease models & mechanisms · 2022Article
- Identification of Disease-Associated Cryptococcal Proteins Reactive With Serum IgG From Cryptococcal Meningitis Patients.Frontiers in immunology · 2021Article
- Controlled Transcription of Regulator GeneMicroorganisms · 2020Article
- The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes.The Journal of antibiotics · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFungi are treasure chests for yet unexplored natural products. However, exploitation of their real potential remains difficult as a significant proportion of biosynthetic gene clusters appears silent under standard laboratory conditions. Therefore, elucidation of novel products requires gene activation or heterologous expression. For heterologous gene expression, we previously developed an expression platform in
resultsIn this study, we extended this system by regulating expression of
conclusionThis tightly regulated but strongly inducible expression system enables high level production of secondary metabolites most likely even those with toxic potential. Furthermore, this system is compatible with polycistronic gene expression and, thus, suitable for the discovery of novel natural products.
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