ArticleScientific reports2017
Micro-patterned agarose gel devices for single-cell high-throughput microscopy of E. coli cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Seeing Bacterial Persistence Through Different Experimental Lenses: How Methodology Shapes Biological Interpretation.Pathogens (Basel, Switzerland) · 2026Review
- Mtb-Timer: a fluorescent reporter to visualizemSystems · 2026Article
- From Fabrication to Flow: Impact of Print Orientation on Surface Qualities and Capillary-Driven Flow in Laser SLA-based Open Microchannels.bioRxiv : the preprint server for biology · 2026Article
- Article
- Antimicrobial peptide LL37 is potent against non-growingmSphere · 2025Article
- Open Microfluidic Cell Culture in Hydrogels Enabled by 3D-Printed Molds.Bioengineering (Basel, Switzerland) · 2025Article
- Strength in numbers: Unleashing the potential of trans-scale scope AMATERAS for massive cell quantification.Biophysics and physicobiology · 2024Review
- Quality Control Method and Device for Producing Agarose Micropads.microPublication biology · 2024Article
- High-Efficiency Single-Cell Containment Microdevices Based on Fluid Control.Micromachines · 2023Article
- High-Throughput Gel Microbeads as Incubators for Bacterial Competition Study.Micromachines · 2023Article
- Density fluctuations, homeostasis, and reproduction effects in bacteria.Communications biology · 2022Article
- Mammalian Cell Interaction with Periodic Surface Nanostructures.International journal of molecular sciences · 2022Article
- Effective Cryopreservation of a Bioluminescent AuxotrophicBiosensors · 2021Article
- Layer-by-layer fabrication of 3D hydrogel structures using open microfluidics.Lab on a chip · 2020Article
- Comparison of Escherichia coli surface attachment methods for single-cell microscopy.Scientific reports · 2019Article
- Using fluorescence microscopy to shed light on the mechanisms of antimicrobial peptides.Future medicinal chemistry · 2019Review
- Article
- Promoted Angiogenesis and Osteogenesis by Dexamethasone-loaded Calcium Phosphate Nanoparticles/Collagen Composite Scaffolds with Microgroove Networks.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput microscopy of bacterial cells elucidated fundamental cellular processes including cellular heterogeneity and cell division homeostasis. Polydimethylsiloxane (PDMS)-based microfluidic devices provide advantages including precise positioning of cells and throughput, however device fabrication is time-consuming and requires specialised skills. Agarose pads are a popular alternative, however cells often clump together, which hinders single cell quantitation. Here, we imprint agarose pads with micro-patterned 'capsules', to trap individual cells and 'lines', to direct cellular growth outwards in a straight line. We implement this micro-patterning into multi-pad devices called CapsuleHotel and LineHotel for high-throughput imaging. CapsuleHotel provides ~65,000 capsule structures per mm
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.