ArticlePlant physiology2018
A New Barley Stripe Mosaic Virus Allows Large Protein Overexpression for Rapid Function Analysis.
Article in Plant physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Wheat's Up with CRISPR-Cas-Current Advances, Obstacles and Perspectives.International journal of molecular sciences · 2026Review
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Stripe rust fungus subverts wheat phase separation-mediated immunity to promote infection.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Engineer and split an efficient hypercompact CRISPR-CasΦ genome editor in plants.Plant communications · 2024Article
- Review
- Modulation of the wheat transcriptome by TaZFP13D under well-watered and drought conditions.Plant molecular biology · 2024Article
- Intein-mediated splitFrontiers in genome editing · 2024Article
- Integrating cotyledon-based virus-induced gene silencing with visual marker promises a rapid, highly effective validation of gene functions inFrontiers in plant science · 2024Article
- Genome-Wide Identification and Analysis of theInternational journal of molecular sciences · 2023Article
- Rescue of the first alphanucleorhabdovirus entirely from cloned complementary DNA: An efficient vector for systemic expression of foreign genes in maize and insect vectors.Molecular plant pathology · 2023Article
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Frontiers in plant science · 2023Article
- A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus.Plants (Basel, Switzerland) · 2022Article
- VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants.Stress biology · 2022Review
- Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Genome-wide identification of the BASS gene family in four Gossypium species and functional characterization of GhBASSs against salt stress.Scientific reports · 2021Article
- Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus.BMC plant biology · 2021Article
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
- The barley stripe mosaic virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance.BMC plant biology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the genetic and molecular bases of gene function is of increasing importance to harness their potential to produce plants with novel traits. One important objective is the improvement of plant productivity to meet future demands in food crop production. Gene function is mostly characterized through overexpression or silencing in transgenic plants. This approach is a lengthy procedure, especially in cereals. Plant viral expression systems can be used for rapid expression of proteins. However, current systems have a small cargo capacity and have mostly been used for gene silencing. Here, a four-component barley stripe mosaic virus-based system with high cargo capacity was constructed for the rapid and stable expression of recombinant proteins in different plant species, allowing function analyses at different stages of development. Fluorescent marker proteins are expressed at high levels within 1 week, and a proof of efficient function analysis is shown using the aluminum malate transporter1 gene. In addition to the ability of gene cotransformation, this work demonstrates that the four-component barley stripe mosaic virus-based system allows the overexpression of cDNAs of up to 2,100 nucleotides (encoding a protein of ∼78 kD), thereby providing an invaluable tool to accelerate functional genomics and proteomic research in monocot and dicot species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.