Evidence map›Paper›PMID 29268766›Full record

ArticleVirology journal2017

Human papillomavirus detection using the Abbott RealTime high-risk HPV tests compared with conventional nested PCR coupled to high-throughput sequencing of amplification products in cervical smear specimens from a Gabonese female population.

Pamela Moussavou-Boundzanga, Ismaël Hervé Koumakpayi, Ingrid Labouba, Eric M Leroy, Ernest Belembaogo, Nicolas Berthet

Open access · goldAbstract readComparative Study
In one paragraph

Article in Virology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Pamela Moussavou-BoundzangaCentre International de Recherches Médicales de Franceville (CIRMF), Department of Zoonosis and Emerging Diseases, 769, Franceville, BP, Gabon.
Ismaël Hervé KoumakpayiInstitut de Cancérologie de Libreville (ICL), Libreville, Gabon.
Ingrid LaboubaCentre International de Recherches Médicales de Franceville (CIRMF), Department of Zoonosis and Emerging Diseases, 769, Franceville, BP, Gabon.
Eric M LeroyCentre International de Recherches Médicales de Franceville (CIRMF), Department of Zoonosis and Emerging Diseases, 769, Franceville, BP, Gabon.
Ernest BelembaogoInstitut de Cancérologie de Libreville (ICL), Libreville, Gabon.
Nicolas BerthetCentre International de Recherches Médicales de Franceville (CIRMF), Department of Zoonosis and Emerging Diseases, 769, Franceville, BP, Gabon. nicolas.berthet@ird.fr.
Centre International de Recherches Médicales de Franceville · GACancer Institute (WIA) · INCentre National de la Recherche Scientifique · FRLibreville Hospital · GA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCervical cancer is the fourth most common malignancy in women worldwide. However, screening with human papillomavirus (HPV) molecular tests holds promise for reducing cervical cancer incidence and mortality in low- and middle-income countries. The performance of the Abbott RealTime High-Risk HPV test (AbRT) was evaluated in 83 cervical smear specimens and compared with a conventional nested PCR coupled to high-throughput sequencing (HTS) to identify the amplicons.

resultsThe AbRT assay detected at least one HPV genotype in 44.57% of women regardless of the grade of cervical abnormalities. Except for one case, good concordance was observed for the genotypes detected with the AbRT assay in the high-risk HPV category determined with HTS of the amplicon generated by conventional nested PCR.

conclusionsThe AbRT test is an easy and reliable molecular tool and was as sensitive as conventional nested PCR in cervical smear specimens for detection HPVs associated with high-grade lesions. Moreover, sequencing amplicons using an HTS approach effectively identified the genotype of the hrHPV identified with the AbRT test.

Indexed as

AdultAgedAged, 80 and overCervix UteriDNA, ViralFemaleGabonGenotypeHigh-Throughput Nucleotide SequencingHuman papillomavirus 16Human papillomavirus 18HumansMiddle AgedMolecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionPapillomaviridaeDNA, ViralAbbott RealTime high-risk HPVCervical cancerConventional PCRHuman papillomavirusScreening

Identifiers

PMID29268766
PMCPMC5740888
OpenAlexW2781216154

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.