ArticlePloS one2017
Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Polyphenol-polysaccharide interactions: molecular mechanisms and potential applications in food systems - a comprehensive review.Food production, processing and nutrition · 2025Review
- Active pullulan-based coatings incorporated withFood science and biotechnology · 2024Article
- A review of extraction, purification, structural properties and biological activities of legumes polysaccharides.Frontiers in nutrition · 2022Review
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Authors and funding
7 authors.
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Abstract
In this study, the extraction of water-soluble polysaccharides from the seed coat of black soybean (BSCP) was investigated and optimized. A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction temperature, 100°C; ratio of water to material, 22.3 mL/g; and extraction time, 133.2 min. Under these conditions, the experimental yield of polysaccharides was 10.56%, which was consistent with the predictive yield. A novel galactomannan, BSCP-1, with a molecular weight of 7.55 × 105 Da determined by high-performance gel permeation chromatography, was isolated from the black soybean seed coat. Through gas chromatography-mass spectrometry analysis, BSCP-1 was identified as a galactomannan consisting of galactose, mannose and rhamnose in a molar ratio of 6.01:3.56:1.00. Cytotoxicity against the human gastric carcinoma cancer cell line was also determined.
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