ArticleThe Journal of eukaryotic microbiology2018
A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei.
Article in The Journal of eukaryotic microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Application of Double-Inducible Expression System in Bloodstream-Form Trypanosoma brucei.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An independently tunable dual control system for RNAi complementation in Trypanosoma brucei.PloS one · 2025Article
- Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn2+ in protein glycosylation needed for host cell invasion and intracellular replication.PLoS pathogens · 2021Article
- A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression inFrontiers in cellular and infection microbiology · 2020Article
- State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids.The Journal of eukaryotic microbiology · 2019Review
- Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes.The Journal of eukaryotic microbiology · 2018Article
- Simplified inducible system for Trypanosoma brucei.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Generation of conditional mutants in Trypanosoma brucei can be done by the use of RNA interference (RNAi). However, RNAi frequently produces off target effects. Here, we present an alternative strategy in which the glmS ribozyme is inserted in the C-terminal region of one allele of a GOI and effectively knocks it down in response to the presence of glucosamine in the culture medium. Using several endogenous genes, we show that the glmS ribozyme cleaves the mRNA in vivo leading to reduction in mRNA and protein expression following glucosamine treatment in both T. brucei procyclic and bloodstream forms. Glucosamine-induced ribozyme activation can be rapidly reversed by removing the inducer. In summary, the glmS ribozyme could be used as a tool to study essential genes in T. brucei.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.