Evidence map›Paper›PMID 29265183›Full record

ArticleBritish journal of haematology2018

Purification of zebrafish erythrocytes as a means of identifying a novel regulator of haematopoiesis.

Kasem Kulkeaw, Tomoko Inoue, Tohru Ishitani, Yoichi Nakanishi, Leonard I Zon, Daisuke Sugiyama

Open access · bronzeAbstract read
In one paragraph

Article in British journal of haematology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Kasem KulkeawDepartment of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Tomoko InoueDepartment of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Tohru IshitaniDivision of Cell Regulation Systems, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan.
Yoichi NakanishiDepartment of Clinical Study, Centre for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan.
Leonard I ZonDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Daisuke SugiyamaDepartment of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.ORCID 0000-0001-6662-368X
Kyushu University · JPHoward Hughes Medical Institute · US

Funding

TRANSCRIPTION FACTOR GATA-1 IN TERMINAL ERYTHROID DEVELOPMENTP01HL032262 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI ORKIN, STUART H · 1985 to 2021
$38.9M
Project-002U54DK110805 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI ORKIN, STUART H, ZON, LEONARD IRA · 2016 to 2020
$5.9M
EMBRYONIC HEMATOPOIESISR01HL048801 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI ZON, LEONARD IRA · 1992 to 2016
$5.7M
NHLBI NIH HHS P01 HL032262NHLBI NIH HHS R01 HL048801NIDDK NIH HHS U54 DK110805
6 · The paper itself

Abstract

Zebrafish embryos are useful to study haematopoietic gene function in vertebrates, although lack of antibodies to zebrafish proteins has limited the purification of specific cell populations. Here, we purified primitive zebrafish erythrocytes using 1, 5-bis{[2-(di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione (DRAQ5

Indexed as

HematopoiesisAnimalsBiomarkersCell SeparationErythrocytesFlow CytometryGene Expression RegulationImmunophenotypingOrgan SpecificityZebrafishBiomarkersDRAQ5TMflow cytometryprimitive erythrocytezebrafish

Identifiers

PMID29265183
PMCPMC5977992
OpenAlexW2781155814

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.