Evidence map›Paper›PMID 29264804›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4.

Amanda M Nevins, Adriano Marchese

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
10.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Amanda M NevinsDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
Adriano MarcheseDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA. amarchese@mcw.edu.
Medical College of Wisconsin · US

Funding

Role of beta-arrestins in G protein-coupled receptor sorting and signalingR01GM106727 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI MARCHESE, ADRIANO · 2014 to 2022
$2.9M
GPCR regulation of Akt signalingR01GM122889 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI MARCHESE, ADRIANO · 2017 to 2020
$1.3M
NIGMS NIH HHS R01 GM106727NIGMS NIH HHS R01 GM122889NIMHD NIH HHS L60 MD003100
6 · The paper itself

Abstract

G protein-coupled receptors (GPCRs) are cell surface receptors that relay extracellular signals to the inside of the cells. C-X-C chemokine receptor 4 (CXCR4) is a GPCR that undergoes receptor internalization and recycling upon stimulation with its cognate ligand, C-X-C chemokine 12 (CXCL12). Using this receptor/ligand pair we describe the use of two techniques, enzyme-linked immunosorbent assay (ELISA) and flow cytometry, widely used to quantify GPCR internalization from the plasma membrane and its return to the cell surface by recycling.

Indexed as

Absorption, PhysicochemicalAntibodies, MonoclonalCell MembraneChemokine CXCL12Enzyme-Linked Immunosorbent AssayFlow CytometryFluorescent DyesHEK293 CellsHeLa CellsHumansLigandsReceptors, CXCR4Antibodies, MonoclonalChemokine CXCL12CXCL12 protein, humanCXCR4 protein, humanFluorescent DyesLigandsReceptors, CXCR4C-X-C chemokine ligand 12 (CXCL12)C-X-C chemokine receptor 4 (CXCR4)ELISAFlow cytometryG Protein-coupled receptor (GPCR)Receptor internalization

Identifiers

PMID29264804
PMCPMC6345166
OpenAlexW2777506012

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.