ArticleScientific reports2017
Formation of Cell Membrane Component Domains in Artificial Lipid Bilayer.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 35 citations in OpenAlex.
- Two-step formation of multilayered membrane pattern from living cells and probing its nanoscale physicochemical properties via nanopipette.Materials today. Bio · 2026Article
- Emergent properties of melanin-inspired peptide/RNA condensates.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Assembly of Cell-Free Synthesized Ion Channel Molecules in Artificial Lipid Bilayer Observed by Atomic Force Microscopy.Membranes · 2023Article
- Creation of supramolecular biomembrane by the bottom-up self-assembly: Where material science meets biophysics.Biophysics and physicobiology · 2022Article
- Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution.Micromachines · 2021Article
- Hyperphosphatemia and Cardiovascular Disease.Frontiers in cell and developmental biology · 2021Review
- Development of a coarse-grained lipid model, LIME 2.0, for DSPE using multistate iterative Boltzmann inversion and discontinuous molecular dynamics simulations.Fluid phase equilibria · 2020Article
- Soft X-ray absorption spectroscopy in the low-energy region explored using an argon gas window.Journal of synchrotron radiation · 2020Article
- Supported Planar Single and Multiple Bilayer Formation by DOPC Vesicle Rupture on Mica Substrate: A Mechanism as Revealed by Atomic Force Microscopy Study.The Journal of membrane biology · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lipid bilayer environment around membrane proteins strongly affects their structure and functions. Here, we aimed to study the fusion of proteoliposomes (PLs) derived from cultured cells with an artificial lipid bilayer membrane and the distribution of the PL components after the fusion. PLs, which were extracted as a crude membrane fraction from Chinese hamster ovary (CHO) cells, formed isolated domains in a supported lipid bilayer (SLB), comprising phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cholesterol (Chol), after the fusion. Observation with a fluorescence microscope and an atomic force microscope showed that the membrane fusion occurred selectively at microdomains in the PC + PE + Chol-SLB, and that almost all the components of the PL were retained in the domain. PLs derived from human embryonic kidney 293 (HEK) cells also formed isolated domains in the PC + PE + Chol-SLB, but their fusion kinetics was different from that of the CHO-PLs. We attempted to explain the mechanism of the PL-SLB fusion and the difference between CHO- and HEK-PLs, based on a kinetic model. The domains that contained the whole cell membrane components provided environments similar to that of natural cell membranes, and were thus effective for studying membrane proteins using artificial lipid bilayer membranes.
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