ArticleScientific reports2017
Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- Genome-Wide Development and Characterization of Microsatellite Markers in the Great Web-Spinning SawflyEcology and evolution · 2024Article
- A comparative plastome approach enhances the assessment of genetic variation in the Melilotus genus.BMC genomics · 2024Article
- Genome survey sequencing-based SSR marker development and their validation in Dendrocalamus longispathus.Functional & integrative genomics · 2023Article
- Identification and genetic diversity analysis of hybrid offspring of azalea based on EST-SSR markers.Scientific reports · 2022Article
- Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis.Plant biotechnology journal · 2022Article
- Genome-wide development and application of miRNA-SSR markers inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Genome-Wide Identification and Development of LTR Retrotransposon-Based Molecular Markers for thePlants (Basel, Switzerland) · 2021Article
- Characterization, validation, and cross-species transferability of EST-SSR markers developed from Lycoris aurea and their application in genetic evaluation of Lycoris species.BMC plant biology · 2020Article
- Development of EST-SSR markers forPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Development of EST-SSR Markers Linked to Flowering Candidate Genes inPlants (Basel, Switzerland) · 2020Article
- Development of EST-SSR markers and association mapping with floral traits in Syringa oblata.BMC plant biology · 2020Article
- Genome-wide development of miRNA-based SSR markers in Cleistogenes songorica and analysis of their transferability to Gramineae/non-Gramineae species.Journal of applied genetics · 2020Article
- Development of polymorphic EST-SSR markers and their applicability in genetic diversity evaluation in Rhododendron arboreum.Molecular biology reports · 2020Article
- Transcriptome analysis of terpenoid biosynthetic genes and simple sequence repeat marker screening in Eucommia ulmoides.Molecular biology reports · 2020Article
- Article
- Characterization and Development of Microsatellite Markers inFrontiers in genetics · 2020Article
- EST-SSR marker development based on RNA-sequencing of E. sibiricus and its application for phylogenetic relationships analysis of seventeen Elymus species.BMC plant biology · 2019Article
- Article
- Article
- Evaluation of the Genetic Diversity and Differentiation of Black Locust (International journal of molecular sciences · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melilotus is one of the most important legume forages, but the lack of molecular markers has limited the development and utilization of Melilotus germplasm resources. In the present study, 151 M clean reads were generated from various genotypes of Melilotus albus using Illumina sequencing. A total of 19,263 potential EST-SSRs were identified from 104,358 unigene sequences. Moreover, 18,182 primer pairs were successfully designed, and 550 primer pairs were selected using criteria of base repeat type, fragment length and annealing temperature. In addition, 550 primer pairs were screened by using PCR amplification products and used to assess polymorphisms in 15 M. albus accessions. A total of 114 primer pairs were detected as being highly polymorphic, and the average polymorphism information content (PIC) value was 0.79. Furthermore, those 114 polymorphic primer pairs were used to evaluate the transferability to 18 species of the genus Melilotus, and 70 EST-SSR markers were found to be transferable among the 18 Melilotus species. According to the UPGMA dendrogram and STRUCTURE analysis, the 18 Melilotus species were classified into three clusters. This study offers a valuable resource for the genetic diversity and molecular assisted breeding of germplasm resources in the genus Melilotus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.