ArticleParasites & vectors2017
Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Ticks and Tick-Borne Microorganisms in Australian Wildlife: A Scoping One Health Evidence Synthesis of Reported Associations and Knowledge Gaps.Pathogens (Basel, Switzerland) · 2026Review
- New host record of Amblyomma pakhtunensis on the Indian Pangolin (Manis crassicaudata) with detection of a distinct Borrelia lineage.Scientific reports · 2026Article
- Characterization of the family-levelmBio · 2025Article
- Unveiling hidden threats: Molecular surveillance of bacterial and protozoan infections in Asian water monitors (Current research in parasitology & vector-borne diseases · 2025Article
- A distinct Borrelia clade within the relapsing fever group from cat-infesting Haemaphysalis semermis Neumann 1901 (Acari: Ixodidae).Veterinary research communications · 2024Article
- Tick-borne zoonotic flaviviruses andOne health (Amsterdam, Netherlands) · 2024Review
- Detection of tick-borne bacterial DNA (Rickettsia sp.) in reptile ticks Amblyomma moreliae from New South Wales, Australia.Parasitology research · 2024Article
- Characterisation and comparative genomics of three new Varanus-associated Borrelia spp. from Indonesia and Australia.Parasites & vectors · 2023Article
- Unravelling the Diversity of Microorganisms in Ticks from Australian Wildlife.Pathogens (Basel, Switzerland) · 2023Article
- Molecular evidence ofFrontiers in veterinary science · 2023Article
- Molecular Detection of Tick-Borne Bacteria fromMicroorganisms · 2022Article
- Metatranscriptomic profiling reveals diverse tick-borne bacteria, protozoans and viruses in ticks and wildlife from Australia.Transboundary and emerging diseases · 2022Article
- The bacterial biome of ticks and their wildlife hosts at the urban-wildland interface.Microbial genomics · 2021Article
- Reptile vector-borne diseases of zoonotic concern.International journal for parasitology. Parasites and wildlife · 2021Review
- Molecular Detection of NovelPathogens (Basel, Switzerland) · 2021Article
- Parasites of wombats (family Vombatidae), with a focus on ticks and tick-borne pathogens.Parasitology research · 2021Review
- Morphological identification of ticks and molecular detection of tick-borne pathogens from bare-nosed wombats (Vombatus ursinus).Parasites & vectors · 2021Article
- Current debates and advances in tick microbiome research.Current research in parasitology & vector-borne diseases · 2021Review
- Unbiased Characterization of the Microbiome and Virome of Questing Ticks.Frontiers in microbiology · 2021Article
- Biotic Factors Influence Microbiota of Nymph Ticks from Vegetation in Sydney, Australia.Pathogens (Basel, Switzerland) · 2020Article
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Authors and funding
7 authors.
Funding
Abstract
backgroundKnowledge on the capacity of Australian ticks to carry Borrelia species is currently limited or missing. To evaluate the potential of ticks to carry bacterial pathogens and their DNA, it is imperative to have a robust workflow that maximises recovery of bacterial DNA within ticks in order to enable accurate identification. By exploiting the bilateral anatomical symmetry of ticks, we were able to directly compare two DNA extraction methods for 16S rRNA gene diversity profiling and pathogen detection. We aimed to assess which combination of DNA extraction and 16S rRNA hypervariable region enables identification of the greatest bacterial diversity, whilst minimising bias, and providing the greatest capacity for the identification of Borrelia spp.
resultsWe collected Australian endemic ticks (Bothriocroton undatum), isolated DNA from equal tick halves using two commercial DNA extraction methods and sequenced samples using V1-V3 and V3-V4 16S rRNA gene diversity profiling assays. Two distinct Borrelia spp. operational taxonomic units (OTUs) were detected using the V1-V3 16S rRNA hypervariable region and matching Borrelia spp. sequences were obtained using a conventional nested-PCR. The tick 16S rRNA gene diversity profile was dominated by Rickettsia spp. (98-99%), while the remaining OTUs belonged to Proteobacteria (51-81%), Actinobacteria (6-30%) and Firmicutes (2-7%). Multiple comparisons tests demonstrated biases in each of the DNA extraction kits towards different bacterial taxa.
conclusionsTwo distinct Borrelia species belonging to the reptile-associated Borrelia group were identified. Our results show that the method of DNA extraction can promote bias in the final microbiota identified. We determined an optimal DNA extraction method and 16S rRNA gene diversity profile assay that maximises detection of Borrelia species.
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