ArticleBiophysical journal2017
Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.Molecules (Basel, Switzerland) · 2025Review
- Genetically Encoded Reporters to Monitor Hypoxia.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Nanoscale Bilirubin Analysis in Translational Research and Precision Medicine by the Recombinant Protein HUG.International journal of molecular sciences · 2023Article
- Macromolecular and Solution Properties of the Recombinant Fusion Protein HUG.Biomacromolecules · 2022Article
- Genetically Encoded Fluorescent Redox Indicators for Unveiling Redox Signaling and Oxidative Toxicity.Chemical research in toxicology · 2021Review
- Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy.Frontiers in molecular biosciences · 2021Article
- Disrupting Fluorescence by Mutagenesis in a Green Fluorescent Fatty Acid Binding Protein from a Marine Eel.The protein journal · 2020Article
- Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy.Nature communications · 2020Article
- Stable genetic integration of a red fluorescent protein in a virulent Group AAccess microbiology · 2019Article
- Fluorescence enhancement of a ligand-activated fluorescent protein induced by collective noncovalent interactions.Chemical science · 2018Article
- The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs.Genes & diseases · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
UnaG is a recently discovered ligand-induced fluorescent protein that utilizes bound bilirubin (BR) as its fluorophore. The fluorescence of the UnaG-BR complex (holoUnaG) compares in quantum efficiency to that of enhanced green fluorescent protein, but it is superior in that the fluorophore formation is instantaneous and not dependent on oxygen; hence, much attention has been paid to UnaG as a new fluorescent probe. However, many important molecular properties of fluorescent probes remain unknown, such as the association/dissociation rates of BR, which determine the stability thereof, and the dispersibility of UnaG in aqueous solutions, which influence the functions of labeled proteins. In this study, we found, in the process of investigating the association rate, that the holoUnaG takes two distinct fluorescence states, which we named holoUnaG
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.