ArticlePloS one2017
A real-time PCR method for quantification of the total and major variant strains of the deformed wing virus.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Neurotranscriptomic profiling of deformed wing virus-infected honey bee foragers (Apis mellifera) with different cognitive abilities.Biology open · 2025Article
- Mechanical transfer of honey bee (Hymenoptera: Apidae) virus sequences to wax by worker traffic and aerosolization.Journal of insect science (Online) · 2025Article
- Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies.Scientific reports · 2025Article
- Deformed wing virus coopts the host arginine kinase to enhance its fitness in honey bees (Apis mellifera).BMC biology · 2025Article
- Detection and characterization of Deformed Wing Virus (DWV) in apiaries with stationary and migratory management in the province of Entre Ríos, Argentina.Scientific reports · 2024Article
- Learning performance and GABAergic pathway link to deformed wing virus in the mushroom bodies of naturally infected honey bees.The Journal of experimental biology · 2024Article
- Selection and Multiplexing of Reverse Transcription-Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens.Microorganisms · 2024Article
- Complete genome sequence of deformed wing virus and black queen cell virus isolated from honeybees (Microbiology resource announcements · 2024Article
- Article
- Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Journal of veterinary research · 2023Article
- Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication.Frontiers in microbiology · 2023Article
- Virus Prevalence in Egg Samples Collected from Naturally Selected and Traditionally Managed Honey Bee Colonies across Europe.Viruses · 2022Article
- Viral communities in the parasite Varroa destructor and in colonies of their honey bee host (Apis mellifera) in New Zealand.Scientific reports · 2022Article
- Molecular Detection and Differentiation of Arthropod, Fungal, Protozoan, Bacterial and Viral Pathogens of Honeybees.Veterinary sciences · 2022Review
- The Multiplicity of Infection of Recombinant Vaccinia Virus Expressing the T7 RNA Polymerase Determines the Rescue Efficiency of Vesicular Stomatitis Virus.Frontiers in microbiology · 2022Article
- Histopathological Features of Symptomatic and Asymptomatic Honeybees Naturally Infected by Deformed Wing Virus.Pathogens (Basel, Switzerland) · 2021Article
- Codon optimization, expression inPeerJ · 2020Article
- Review
- Multiple Virus Infections in Western Honeybee (Viruses · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
European honey bees (Apis mellifera) are critically important to global food production by virtue of their pollination services but are severely threatened by deformed wing virus (DWV) especially in the presence of the external parasite Varroa destructor. DWV exists as many viral strains with the two major variants (DWV-A and DWV-B) varying in virulence. A single plasmid standard was constructed containing three sections for the specific determination of DWV-A (VP2 capsid region), DWV-B (IRES) and a conserved region suitable for total DWV (helicase region). The assays were confirmed as specific and discriminatory with limits of detections of 25, 25 and 50 genome equivalents for DWV-A, DWV-B and total-DWV, respectively. The methods were successfully tested on Apis mellifera and V. destructor samples with varying DWV profiles. The new method determined a more accurate total DWV titre in samples with substantial DWV-B than the method currently described in the COLOSS Beebook. The proposed assays could be utilized for the screening of large quantities of bee material for both a total DWV load overview along with more detailed investigations into DWV-A and DWV-B profiles.
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