ArticlePloS one2017
Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 73 citations in OpenAlex.
- Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae).Pest management science · 2026Article
- Evaluation of CPP and LDH nanocarriers for dsRNA efficiency inFrontiers in insect science · 2026Article
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- Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.Functional & integrative genomics · 2023Review
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- Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil.Frontiers in molecular biosciences · 2023Article
- dsRNase1 contribution to dsRNA degradation activity in the Sf9 cells conditioned medium.Frontiers in insect science · 2023Article
- Host-induced silencing of the CpCHI gene resulted in developmental abnormalities and mortality in maize stem borer (Chilo partellus).PloS one · 2023Article
- Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (International journal of molecular sciences · 2022Article
- Identification of the Extracellular Nuclease Influencing Soaking RNA Interference Efficiency inInternational journal of molecular sciences · 2022Article
- Localized efficacy of environmental RNAi in Tetranychus urticae.Scientific reports · 2022Article
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides.3 Biotech · 2021Review
- Dissecting protein domain variability in the core RNA interference machinery of five insect orders.RNA biology · 2021Article
- Article
- Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil.Neotropical entomology · 2021Article
- Highly Variable Dietary RNAi Sensitivity Among Coleoptera.Frontiers in plant science · 2021Review
- Advances in exogenous RNA delivery techniques for RNAi-mediated pest control.Molecular biology reports · 2020Review
- Insecticidal Gene Silencing by RNAi in the Neotropical Region.Neotropical entomology · 2020Review
- Enhancing RNAi Efficiency to Decipher the Functional Response of Potential Genes inFrontiers in physiology · 2020Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) approaches have been applied as a biotechnological tool for controlling plant insect pests via selective gene down regulation. However, the inefficiency of RNAi mechanism in insects is associated with several barriers, including dsRNA delivery and uptake by the cell, dsRNA interaction with the cellular membrane receptor and dsRNA exposure to insect gut nucleases during feeding. The cotton boll weevil (Anthonomus grandis) is a coleopteran in which RNAi-mediated gene silencing does not function efficiently through dsRNA feeding, and the factors involved in the mechanism remain unknown. Herein, we identified three nucleases in the cotton boll weevil transcriptome denoted AgraNuc1, AgraNuc2, and AgraNuc3, and the influences of these nucleases on the gene silencing of A. grandis chitin synthase II (AgraChSII) were evaluated through oral dsRNA feeding trials. A phylogenetic analysis showed that all three nucleases share high similarity with the DNA/RNA non-specific endonuclease family of other insects. These nucleases were found to be mainly expressed in the posterior midgut region of the insect. Two days after nuclease RNAi-mediated gene silencing, dsRNA degradation by the gut juice was substantially reduced. Notably, after nucleases gene silencing, the orally delivered dsRNA against the AgraChSII gene resulted in improved gene silencing efficiency when compared to the control (non-silenced nucleases). The data presented here demonstrates that A. grandis midgut nucleases are effectively one of the main barriers to dsRNA delivery and emphasize the need to develop novel RNAi delivery strategies focusing on protecting the dsRNA from gut nucleases and enhancing its oral delivery and uptake to crop insect pests.
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