ArticleReproductive medicine and biology2017
Immobilized pH in culture reveals an optimal condition for somatic cell reprogramming and differentiation of pluripotent stem cells.
Article in Reproductive medicine and biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Scalable Production of Extracellular Vesicles and Its Therapeutic Values: A Review.International journal of molecular sciences · 2022Review
- In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture.Communications biology · 2022Article
- A prevalent neglect of environmental control in mammalian cell culture calls for best practices.Nature biomedical engineering · 2021Article
- Response of Pluripotent Stem Cells to Environmental Stress and Its Application for Directed Differentiation.Biology · 2021Review
- pH variation impacts molecular pathways associated with somatic cell reprogramming and differentiation of pluripotent stem cells.Reproductive medicine and biology · 2021Review
- Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells.World journal of stem cells · 2018Article
- Gas Partial Pressure in Cultured Cells: Patho-Physiological Importance and Methodological Approaches.Frontiers in physiology · 2018Review
- Human Pluripotent Stem Cell Culture: Current Status, Challenges, and Advancement.Stem cells international · 2018Review
- Immobilized pH in culture reveals an optimal condition for somatic cell reprogramming and differentiation of pluripotent stem cells.Reproductive medicine and biology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aim: One of the parameters that greatly affects homeostasis in the body is the pH. Regarding reproductive biology, germ cells, such as oocytes or sperm, are exposed to severe changes in pH, resulting in dramatic changes in their characteristics. To date, the effect of the pH has not been investigated regarding the reprogramming of somatic cells and the maintenance and differentiation of pluripotent stem cells. Methods: In order to investigate the effects of the pH on cell culture, the methods to produce induced pluripotent stem cells (iPSCs) and to differentiate embryonic stem cells (ESCs) into mesendoderm and neuroectoderm were performed at each medium pH from 6.6 to 7.8. Using the cells of the Results: The colony formation rate and timing of the reprogramming of the somatic cells varied depending on the pH of the culture medium. In addition, mesendodermal differentiation of the mouse ESCs was enhanced at the high pH level of 7.8. Conclusion: These results suggest that the pH in the culture medium is one of the key factors in the induction of the reprogramming of somatic cells and in the differentiation of pluripotent stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.