ArticleMethods (San Diego, Calif.)2018
Assessing multiparametric drug response in tissue engineered tumor microenvironment models.
Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Modeling Immunity In Vitro: Slices, Chips, and Engineered Tissues.Annual review of biomedical engineering · 2021Pooled it
- Evaluating Alternatives to Fetal Bovine Serum in the Development of Advanced Biomaterial-Based Tumor Models: Overcoming Challenges in Biofabrication.Bioengineering (Basel, Switzerland) · 2026Article
- Integrated Experimental-Computational Framework for Drug Transport Quantification in 3DMicromachines · 2026Article
- Ex Vivo Model of Breast Cancer Cell Invasion in Live Lymph Node Tissue.ACS pharmacology & translational science · 2025Article
- Ex vivo model of breast cancer cell invasion in live lymph node tissue.bioRxiv : the preprint server for biology · 2024Article
- Development of a Synthetic, Injectable Hydrogel to Capture Residual Glioblastoma and Glioblastoma Stem-Like Cells with CXCL12-Mediated Chemotaxis.Advanced healthcare materials · 2023Article
- In Vitro Models of Blood and Lymphatic Vessels-Connecting Tissues and Immunity.Advanced biology · 2023Review
- Modeling lymphangiogenesis: Pairing in vitro and in vivo metrics.Microcirculation (New York, N.Y. : 1994) · 2023Review
- New tools for immunologists: models of lymph node function from cells to tissues.Frontiers in immunology · 2023Review
- Engineering complexity in human tissue models of cancer.Advanced drug delivery reviews · 2022Review
- Designing Patient-Driven, Tissue-Engineered Models of Primary and Metastatic Breast Cancer.Bioengineering (Basel, Switzerland) · 2022Article
- Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy.Frontiers in oncology · 2022Article
- Three-DimensionalFrontiers in oncology · 2020Article
- Applicability of drug response metrics for cancer studies using biomaterials.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Assessing drug response in engineered brain microenvironments.Brain research bulletin · 2019Review
- Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line.Brain sciences · 2019Article
- Convective forces increase CXCR4-dependent glioblastoma cell invasion in GL261 murine model.Scientific reports · 2018Article
- The human microglial HMC3 cell line: where do we stand? A systematic literature review.Journal of neuroinflammation · 2018Review
- MRI analysis to map interstitial flow in the brain tumor microenvironment.APL bioengineering · 2018Article
- Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression.BMC cancer · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The tumor microenvironment is important in promoting treatment resistance of tumor cells via multiple mechanisms. However, studying this interaction often proves difficult. In vivo animal models are costly, time-consuming, and often fail to adequately predict human response to treatment. Conversely, testing drug response on human tumor cells in vitro in 2D cell culture excludes the important contribution of stromal cells and biophysical forces seen in the in vivo tumor microenvironment. Here, we present tissue-engineered models of both human brain and breast tumor microenvironments incorporating key stromal cell populations for assessing multiple mechanisms of therapeutic response using flow cytometry. We show our physiologically-relevant systems used to interrogate a variety of parameters associated with chemotherapeutic efficacy, including cell death, proliferation, drug uptake, and invasion of cancer and stromal cell populations. The use of flow cytometry allows for single cell, quantitative, and fast assessments of multiple outcomes affecting anti-tumor therapy failure. Our system can be modified to add and remove cellular components with ease, thereby enabling the study of individual cellular contributions in the tumor microenvironment. Together, our models and analysis methods illustrate the importance of developing fast, cost-effective, and reproducible methods to model complex human systems in a physiologically-relevant manner that may prove useful for drug screening efforts in the future.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.