ArticleTicks and tick-borne diseases2018
A molecular algorithm to detect and differentiate human pathogens infecting Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae).
Article in Ticks and tick-borne diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 45 citations in OpenAlex.
- Filling the Gap: Establishing a Statewide Tick and Tick-Borne Pathogen Surveillance Program.Insects · 2026Article
- Community-engaged passive tick surveillance highlights the diversity of ticks and tick-borne pathogens in Illinois.PloS one · 2026Article
- Estimated density of Borrelia burgdorferi sensu stricto-infected Ixodes scapularis nymphs in the eastern United States.Parasites & vectors · 2025Article
- TICKHUNTER: A Targeted Hybridization-Capture Sequencing Approach for the Detection and Characterization of Tick-borne Pathogens and Blood Meals.bioRxiv : the preprint server for biology · 2025Article
- A NovelPathogens (Basel, Switzerland) · 2025Article
- Statewide surveillance of tick-borne pathogens in ticks collected in Delaware using novel multiplex PCR assays.International journal for parasitology. Parasites and wildlife · 2025Article
- Expanding range of Ixodes scapularis Say (Acari: Ixodidae) and Borrelia burgdorferi infection in North Carolina counties, 2018-2023.PloS one · 2025Article
- Geographic variation in the distribution of Anaplasma phagocytophilum variants in host-seeking Ixodes scapularis nymphs and adults in the eastern United States elucidated using next generation sequencing.Ticks and tick-borne diseases · 2024Article
- A next generation sequencing assay combining Ixodes species identification with pathogen detection to support tick surveillance efforts in the United States.Ticks and tick-borne diseases · 2024Article
- Article
- Release the hens: a study on the complexities of guinea fowl as tick control.Journal of medical entomology · 2024Article
- Detection of Borrelia burgdorferi sensu lato species in host-seeking Ixodes species ticks in the United States.Ticks and tick-borne diseases · 2024Article
- Invasive Haemaphysalis longicornis (Acari: Ixodidae) investigation in South Carolina: new records of establishment, pathogen prevalence, and blood meal analyses.Journal of medical entomology · 2023Article
- Prevalence of five human pathogens in host-seeking Ixodes scapularis and Ixodes pacificus by region, state, and county in the contiguous United States generated through national tick surveillance.Ticks and tick-borne diseases · 2023Article
- A bioinformatics pipeline for a tick pathogen surveillance multiplex amplicon sequencing assay.Ticks and tick-borne diseases · 2023Article
- Deer management generally reduces densities of nymphal Ixodes scapularis, but not prevalence of infection with Borrelia burgdorferi sensu stricto.Ticks and tick-borne diseases · 2023Article
- A serological assay to detect and differentiate rodent exposure to soft tick and hard tick relapsing fever infections in the United States.Ticks and tick-borne diseases · 2023Article
- Effects of Neighborhood-Scale Acaricidal Treatments on Infection Prevalence of Blacklegged Ticks (Pathogens (Basel, Switzerland) · 2023Article
- Predicting distributions of blacklegged ticks (Ixodes scapularis), Lyme disease spirochetes (Borrelia burgdorferi sensu stricto) and human Lyme disease cases in the eastern United States.Ticks and tick-borne diseases · 2022Article
- Spatial Heterogeneity of Sympatric Tick Species and Tick-Borne Pathogens Emphasizes the Need for Surveillance for Effective Tick Control.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The incidence and geographic range of tick-borne illness associated with Ixodes scapularis and Ixodes pacificus have dramatically increased in recent decades. Anaplasmosis, babesiosis, and Borrelia spirochete infections, including Lyme borreliosis, account for tens of thousands of reported cases of tick-borne disease every year. Assays that reliably detect pathogens in ticks allow investigators and public health agencies to estimate the geographic distribution of human pathogens, assess geographic variation in their prevalence, and evaluate the effectiveness of prevention strategies. As investigators continue to describe new species within the Borrelia burgdorferi sensu lato complex and to recognize some Ixodes-borne Borrelia species as human pathogens, assays are needed to detect and differentiate these species. Here we describe an algorithm to detect and differentiate pathogens in unfed I. scapularis and I. pacificus nymphs including Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi sensu stricto, Borrelia mayonii, and Borrelia miyamotoi. The algorithm comprises 5 TaqMan real-time polymerase chain reaction assays and 3 sequencing protocols. It employs multiple targets for each pathogen to optimize specificity, a gene target for I. scapularis and I. pacificus to verify tick-derived DNA quality, and a pan-Borrelia target to detect Borrelia species that may emerge as human disease agents in the future. We assess the algorithm's sensitivity, specificity, and performance on field-collected ticks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.