Evidence map›Paper›PMID 29258486›Full record

ArticleBMC biotechnology2017

Laser-fabricated cell patterning stencil for single cell analysis.

Jacob J Messner, Honor L Glenn, Deirdre R Meldrum

Abstract read
In one paragraph

Article in BMC biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jacob J MessnerOSIsoft, 1600 Alvarado St, San Leandro, CA, 94577, USA.
Honor L GlennBiodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ, 85287, USA.ORCID http://orcid.org/0000-0002-8148-4224
Deirdre R MeldrumCenter for Biosignatures Discovery Automation, The Biodesign Institute, Arizona State University, 1001 S. McAllister Ave., P.O. Box 877101, Tempe, AZ, 85287-7101, USA. Deirdre.Meldrum@asu.edu.

Funding

Live-cell Microarray for high-throughput observation of metabolic signaturesU01CA164250 · NCI · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI MELDRUM, DEIRDRE R. · 2011 to 2013
$2.1M
National Institutes of Health U01CA164250NCI NIH HHS U01 CA164250
6 · The paper itself

Abstract

Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.

Indexed as

Cell Culture TechniquesSingle-Cell AnalysisCell Line, TumorCell Physiological PhenomenaHumansLasersMicrofluidic Analytical TechniquesPolymersTissue EngineeringPolymersAdhesionCell cultureCell patterningMicrowellSingle-cell

Identifiers

PMID29258486
PMCPMC5735507

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.