ArticleBMC genomics2017
A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep.International journal of molecular sciences · 2022Pooled it
- A comparison of normalization methods for the expression of genes associated with oxidative stress in the liver of sheep.BMC genomic data · 2025Article
- Comparative transcriptome analysis reveals genes associated with variation in liver copper concentration in Polish Merino sheep.Scientific reports · 2025Article
- Developmental programming: Sex-specific effects of prenatal exposure to a real-life mixture of environmental chemicals on liver function and transcriptome in sheep.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Risk of fragility fracture is aggravated during bone regeneration processes in osteoporotic sheep.PloS one · 2025Article
- Influence of N- and/or P-restriction on bone metabolism in young goats.The British journal of nutrition · 2024Article
- Effects of dietary nitrogen and/or phosphorus reduction on mineral homeostasis and regulatory mechanisms in young goats.Frontiers in veterinary science · 2024Article
- Modulation of GCN2/eIF2α/ATF4 Pathway in the Liver and Induction of FGF21 in Young Goats Fed a Protein- and/or Phosphorus-Reduced Diet.International journal of molecular sciences · 2023Article
- Expression of the pleiotrophin-midkine axis in a sheep tooth socket model of bone healing.Journal of periodontal research · 2023Article
- Article
- Validation of reference genes for whole blood gene expression analysis in cord blood of preterm and full-term neonates and peripheral blood of healthy adults.BMC genomics · 2021Article
- Optimizations for identifying reference genes in bone and cartilage bioengineering.BMC biotechnology · 2021Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- 3D encapsulation and inflammatory licensing of mesenchymal stromal cells alter the expression of common reference genes used in real-time RT-qPCR.Biomaterials science · 2020Article
- Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture.Scientific reports · 2020Article
- Adult ovine chondrocytes in expansion culture adopt progenitor cell properties that are favorable for cartilage tissue engineering.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Artificial intelligence, osteoporosis and fragility fractures.Current opinion in rheumatology · 2019Review
- Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.Molecular biology reports · 2018Article
- Identification of endogenous normalizing genes for expression studies in inguinal ring tissue for scrotal hernias in pigs.PloS one · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundIn order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D low diet, application of steroids, or a combination of these treatments. Transcriptional studies can be performed by applying quantitative real time PCR (RT-qPCR). RT-qPCR estimates mRNA-levels of target genes in relation to reference genes. A chosen set of reference genes should not show variation under experimental conditions. Currently, no standard reference genes are accepted for all tissue types and experimental conditions. Studies examining reference genes for sheep are rare and only one study described stable reference in mandibular bone. However, this type of bone differs from trabecular bone where most osteoporotic fractures occur. The present study aimed at identifying a set of reference genes for relative quantification of transcriptional activity of ovine spine bone and ovine in vitro differentiated mesenchymal stromal cells (MSC) for reliable comparability.
methodsTwelve candidate reference genes belonging to different functional classes were selected and their expression was measured from cultured ovMSCs (n = 18) and ovine bone samples (n = 16), respectively. RefFinder was used to rank the candidate genes.
resultsWe identified B2M, GAPDH, RPL19 and YWHAZ as the best combination of reference genes for normalization of RT-qPCR results for transcriptional analyses of these ovine samples.
conclusionThis study demonstrates the importance of applying a set of reference genes for RT-qPCR analysis in sheep. Based on our data we recommend using four identified reference genes for relative quantification of gene expression studies in ovine bone or for in vitro experiments with osteogenically differentiated ovine MSCs.
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