ArticleThe Journal of cell biology1989
Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 127 citations in OpenAlex.
- Vimentin Intermediate Filaments: A Paradigm Shift From Static Structure to Dynamic Cytoplasmic Network.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Cytoskeletal Proteins and Alzheimer's Disease Pathogenesis: Focusing on the Interplay with Tau Pathology.Biomolecules · 2025Review
- Interaction of small heat shock proteins with light component of neurofilaments (NFL).Cell stress & chaperones · 2017Article
- Neurofilaments and Neurofilament Proteins in Health and Disease.Cold Spring Harbor perspectives in biology · 2017Review
- Topographic regulation of neuronal intermediate filaments by phosphorylation, role of peptidyl-prolyl isomerase 1: significance in neurodegeneration.Histochemistry and cell biology · 2013Review
- Flow birefringence property of desmin filaments.Biophysics (Nagoya-shi, Japan) · 2012Article
- Neurofilament heavy chain expression reveals a unique parasagittal stripe topography in the mouse cerebellum.Cerebellum (London, England) · 2011Article
- Article
- Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing.The Journal of cell biology · 2009Article
- Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration.Molecular neurobiology · 2008Review
- Protein-protein interactions between lens vimentin and alphaB-crystallin using FRET acceptor photobleaching.Molecular vision · 2008Article
- Developmental changes in the neuronal protein composition: a study by high resolution 2D-gel electrophoresis.Molecular and cellular biochemistry · 2005Article
- Tubulin and neurofilament proteins are transported differently in axons of chicken motoneurons.Cellular and molecular neurobiology · 2000Article
- Intermediate filaments in the nervous system: implications in cancer.Cancer metastasis reviews · 1996Review
- Ca(2+)-mediated phosphorylation and proteolysis activity associated with the cytoskeletal fraction from cerebral cortex of rats.Neurochemical research · 1996Article
- The function of intermediate filaments in cell shape and cytoskeletal integrity.The Journal of cell biology · 1996Article
- Effects of brain ischemia on intermediate filaments of rat hippocampus.Neurochemical research · 1996Article
- Cytoskeletal-associated protein kinase and phosphatase activities from cerebral cortex of young rats.Neurochemical research · 1995Article
- Differential dynamics of neurofilament-H protein and neurofilament-L protein in neurons.The Journal of cell biology · 1994Article
- Phosphorylation of native and reassembled neurofilaments composed of NF-L, NF-M, and NF-H by the catalytic subunit of cAMP-dependent protein kinase.Molecular biology of the cell · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have explored the dynamics of intermediate filament assembly and subunit exchange using fluorescently labeled neurofilament proteins and a fluorescence resonance energy transfer assay. Neurofilaments (NFs) are assembled from three highly phosphorylated proteins with molecular masses of 180 (NF-H), 130 (NF-M), and 66 kD (NF-L) of which NF-L forms the structural core. The core component, NF-L, was stoichiometrically labeled at cysteine 321 with fluorescein, coumarin, or biotin-maleimide to produce assembly-competent fluorescent or biotinylated derivatives, respectively. Using coumarin-labeled NF-L as fluorescence donor and fluorescein-labeled NF-L as the fluorescence acceptor, assembly of NF filaments was induced by rapidly raising the NaCl concentration to 170 mM, and the kinetics was followed by the decrease in the donor fluorescence. Assembly of NF-L subunits into filaments does not require nucleotide binding or hydrolysis but is strongly dependent on ionic strength, pH, and temperature. The critical concentration of NF-L, that concentration that remains unassembled at equilibrium with fully formed filaments, is 38 micrograms/ml or 0.6 microM. Under physiological salt conditions NF-L filaments also undergo extensive subunit exchange. Kinetic analysis and evaluation of several possible mechanisms indicate that subunit exchange is preceded by dissociation of subunits from the filament and generation of a kinetically active pool of soluble subunits. Given the concentration of NF-L found in nerve cells and the possibility of regulating this pool, these results provide the first information that intermediate filaments are dynamic structures and that NF-L within the NF complex is in dynamic equilibrium with a small but kinetically active pool of unassembled NF-L units.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.