ArticleThe Journal of cell biology1989
Microtubule polarities indicate that nucleation and capture of microtubules occurs at cell surfaces in Drosophila.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 98 citations in OpenAlex.
- Spatial arrangement, polarity, and posttranslational modifications of the microtubule system in the Drosophila eye.Cell and tissue research · 2024Article
- Focal adhesion-mediated cell anchoring and migration: from in vitro to in vivo.Development (Cambridge, England) · 2022Article
- Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells.Current biology : CB · 2022Article
- Cyst formation in proximal renal tubules caused by dysfunction of the microtubule minus-end regulator CAMSAP3.Scientific reports · 2021Article
- Review
- Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Molecular biology of the cell · 2018Article
- Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids.Journal of visualized experiments : JoVE · 2017Article
- Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its redeployment to non-centrosomal microtubule organizing centres.Open biology · 2017Article
- Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia.Journal of cell science · 2016Article
- The Seckel syndrome and centrosomal protein Ninein localizes asymmetrically to stem cell centrosomes but is not required for normal development, behavior, or DNA damage response in Drosophila.Molecular biology of the cell · 2016Article
- CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Effect of prazosin on diabetic nephropathy patients with positive α1-adrenergic receptor autoantibodies and refractory hypertension.Experimental and therapeutic medicine · 2015Article
- Review
- Intermediate filaments: a role in epithelial polarity.Experimental cell research · 2007Review
- GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells.Molecular biology of the cell · 2007Article
- Assembly of centrosomal proteins and microtubule organization depends on PCM-1.The Journal of cell biology · 2002Article
- The adenomatous polyposis coli protein unambiguously localizes to microtubule plus ends and is involved in establishing parallel arrays of microtubule bundles in highly polarized epithelial cells.The Journal of cell biology · 2002Article
- Centriolar satellites: molecular characterization, ATP-dependent movement toward centrioles and possible involvement in ciliogenesis.The Journal of cell biology · 1999Article
- Nucleation and capture of large cell surface-associated microtubule arrays that are not located near centrosomes in certain cochlear epithelial cells.Journal of anatomy · 1998Article
- Orientation of spindle axis and distribution of plasma membrane proteins during cell division in polarized MDCKII cells.The Journal of cell biology · 1994Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hook decoration with pig brain tubulin was used to assess the polarity of microtubules which mainly have 15 protofilaments in the transcellular bundles of late pupal Drosophila wing epidermal cells. The microtubules make end-on contact with cell surfaces. Most microtubules in each bundle exhibited a uniform polarity. They were oriented with their minus ends associated with their hemidesmosomal anchorage points at the apical cuticle-secreting surfaces of the cells. Plus ends were directed towards, and were sometimes connected to, basal attachment desmosomes at the opposite ends of the cells. The orientation of microtubules at cell apices, with minus ends directed towards the cell surface, is opposite to the polarity anticipated for microtubules which have elongated centrifugally from centrosomes. It is consistent, however, with evidence that microtubule assembly is nucleated by plasma membrane-associated sites at the apical surfaces of the cells (Mogensen, M. M., and J. B. Tucker. 1987. J. Cell Sci. 88:95-107) after these cells have lost their centriole-containing, centrosomal, microtubule-organizing centers (Tucker, J. B., M. J. Milner, D. A. Currie, J. W. Muir, D. A. Forrest, and M.-J. Spencer. 1986. Eur. J. Cell Biol. 41:279-289). Our findings indicate that the plus ends of many of these apically nucleated microtubules are captured by the basal desmosomes. Hence, the situation may be analogous to the polar-nucleation/chromosomal-capture scheme for kinetochore microtubule assembly in mitotic and meiotic spindles. The cell surface-associated nucleation-elongation-capture mechanism proposed here may also apply during assembly of transcellular microtubule arrays in certain other animal tissue cell types.
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