ArticleScientific data2017
Long-read sequencing of the human cytomegalovirus transcriptome with the Pacific Biosciences RSII platform.
Article in Scientific data, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Mapping the temporal transcriptomic signature of a viral pathogen through CAGE and nanopore sequencing.PloS one · 2025Article
- Targeted DNA-seq and RNA-seq of Reference Samples with Short-read and Long-read Sequencing.Scientific data · 2024Article
- KSHV 3.0: a state-of-the-art annotation of the Kaposi's sarcoma-associated herpesvirus transcriptome using cross-platform sequencing.mSystems · 2024Article
- Identification of herpesvirus transcripts from genomic regions around the replication origins.Scientific reports · 2023Article
- KSHV 3.0: A State-of-the-Art Annotation of the Kaposi's Sarcoma-Associated Herpesvirus Transcriptome Using Cross-Platform Sequencing.bioRxiv : the preprint server for biology · 2023Article
- Hybrid sequencing discloses unique aspects of the transcriptomic architecture in equid alphaherpesvirus 1.Heliyon · 2023Article
- Long-read sequencing reveals complex patterns of wraparound transcription in polyomaviruses.PLoS pathogens · 2022Article
- Dual isoform sequencing reveals complex transcriptomic and epitranscriptomic landscapes of a prototype baculovirus.Scientific reports · 2022Article
- Integrative profiling of Epstein-Barr virus transcriptome using a multiplatform approach.Virology journal · 2022Article
- Article
- Time-Course Transcriptome Profiling of a Poxvirus Using Long-Read Full-Length Assay.Pathogens (Basel, Switzerland) · 2021Article
- Combined nanopore and single-molecule real-time sequencing survey of human betaherpesvirus 5 transcriptome.Scientific reports · 2021Article
- Time-course transcriptome analysis of host cell response to poxvirus infection using a dual long-read sequencing approach.BMC research notes · 2021Article
- Combined Short and Long-Read Sequencing Reveals a Complex Transcriptomic Architecture of African Swine Fever Virus.Viruses · 2021Article
- Dynamic Transcriptome Sequencing of Bovine Alphaherpesvirus Type 1 and Host Cells Carried Out by a Multi-Technique Approach.Frontiers in genetics · 2021Article
- SMRT sequencing of full-length transcriptome of birch-leaf pear (Journal of genetics · 2021Article
- Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing.Scientific reports · 2020Article
- Long-read assays shed new light on the transcriptome complexity of a viral pathogen.Scientific reports · 2020Article
- Short and Long-Read Sequencing Survey of the Dynamic Transcriptomes of African Swine Fever Virus and the Host Cells.Frontiers in genetics · 2020Article
- Template-switching artifacts resemble alternative polyadenylation.BMC genomics · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
Long-read RNA sequencing allows for the precise characterization of full-length transcripts, which makes it an indispensable tool in transcriptomics. The human cytomegalovirus (HCMV) genome has been first sequenced in 1989 and although short-read sequencing studies have uncovered much of the complexity of its transcriptome, only few of its transcripts have been fully annotated. We hereby present a long-read RNA sequencing dataset of HCMV infected human lung fibroblast cells sequenced by the Pacific Biosciences RSII platform. Seven SMRT cells were sequenced using oligo(dT) primers to reverse transcribe poly(A)-selected RNA molecules and one library was prepared using random primers for the reverse transcription of the rRNA-depleted sample. Our dataset contains 122,636 human and 33,086 viral (HMCV strain Towne) reads. The described data include raw and processed sequencing files, and combined with other datasets, they can be used to validate transcriptome analysis tools, to compare library preparation methods, to test base calling algorithms or to identify genetic variants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.