ArticleAnalytical and bioanalytical chemistry2018
Two complementary reversed-phase separations for comprehensive coverage of the semipolar and nonpolar metabolome.
Article in Analytical and bioanalytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women.Science (New York, N.Y.) · 2021Trial
- Performance evaluation of polarity switching for plasma untargeted metabolomics using Orbitrap mass spectrometer.Analytical and bioanalytical chemistry · 2026Article
- Low-Temperature HILIC Provides Enhanced Separations and Stability for LC-MS-Based Metabolomics.Journal of proteome research · 2026Article
- Mapping the unknown: an anchor-based molecular networking workflow for comprehensive identification of new psychoactive substances and analogues.Analytical and bioanalytical chemistry · 2026Article
- Multiclass Assays for Measuring Environmental Chemical Mixture Exposure: Analytical Methodologies and Applications in Exposomics Research.Metabolites · 2025Review
- Coordinated Targeting of S6K1/2 and AXL Disrupts Pyrimidine Biosynthesis in PTEN-Deficient Glioblastoma.Cancer research communications · 2024Article
- A Solid-Phase Microextraction-Liquid Chromatography-Mass Spectrometry Method for Analyzing Serum Lipids in Psoriatic Disease.Metabolites · 2023Article
- Article
- Recent Advances in Understanding of Alzheimer's Disease Progression through Mass Spectrometry-Based Metabolomics.Phenomics (Cham, Switzerland) · 2022Article
- Quantification of Fosfomycin in Combination with Nine Antibiotics in Human Plasma and Cation-Adjusted Mueller-Hinton II Broth via LCMS.Antibiotics (Basel, Switzerland) · 2022Article
- Areas of Metabolomic Exploration for Helminth Infections.ACS infectious diseases · 2021Article
- Liquid Chromatography-Mass Spectrometry for Clinical Metabolomics: An Overview.Advances in experimental medicine and biology · 2021Article
- A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Isomeric Differentiation and Acidic Metabolite Identification by Piperidine-Based Tagging, LC-MS/MS, and Understanding of the Dissociation Chemistries.Analytical chemistry · 2020Article
- Chemical Discovery in the Era of Metabolomics.Journal of the American Chemical Society · 2020Review
- A Protocol to Compare Methods for Untargeted Metabolomics.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Trace Phosphate Improves ZIC-pHILIC Peak Shape, Sensitivity, and Coverage for Untargeted Metabolomics.Journal of proteome research · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Although it is common in untargeted metabolomics to apply reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) methods that have been systematically optimized for lipids and central carbon metabolites, here we show that these established protocols provide poor coverage of semipolar metabolites because of inadequate retention. Our objective was to develop an RPLC approach that improved detection of these metabolites without sacrificing lipid coverage. We initially evaluated columns recently released by Waters under the CORTECS line by analyzing 47 small-molecule standards that evenly span the nonpolar and semipolar ranges. An RPLC method commonly used in untargeted metabolomics was considered a benchmarking reference. We found that highly nonpolar and semipolar metabolites cannot be reliably profiled with any single method because of retention and solubility limitations of the injection solvent. Instead, we optimized a multiplexed approach using the CORTECS T3 column to analyze semipolar compounds and the CORTECS C
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.