ArticleWorld journal of microbiology & biotechnology2017
Cloning, purification and characterization of novel Cu-containing nitrite reductase from the Bacillus firmus GY-49.
Article in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 48 citations in OpenAlex.
- A Novel Nitrite Reductase fromBiomolecules · 2025Article
- Article
- Effect and potential mechanism of nitrite reductase B on nitrite degradation byCurrent research in food science · 2024Article
- Novel gene similar to nitrite reductase (NO forming) plays potentially important role in the latency of tuberculosis.Scientific reports · 2021Article
- Article
- Heterologous and Homologous Expression of Proteins from Haloarchaea: Denitrification as Case of Study.International journal of molecular sciences · 2019Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrite is generated from the nitrogen cycle and its accumulation is harmful to environment and it can be reduced to nitric oxid by nitrite reductase. A novel gene from Bacillus firmus GY-49 is identified as a nirK gene encoding Cu-containing nitrite reductase by genome sequence. The full-length protein included a putative signal peptide of 26 amino acids and shown 72.73% similarity with other Cu-containing nitrite reductase whose function was verified. The 993-bp fragment encoding the mature peptide of NirK was cloned into pET-28a (+) vector and overexpressed as an active protein of 36.41 kDa in the E.coli system. The purified enzyme was green in the oxidized state and displayed double gentle peaks at 456 and 608 nm. The specific activity of purified enzyme was 98.4 U/mg toward sodium nitrite around pH 6.5 and 35 °C. The K
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