ArticleNature communications2017
Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 72 citations in OpenAlex.
- Protocol for isotype-resolved 3D imaging and quantification of bone marrow plasma cells in murine femurs.STAR protocols · 2026Article
- Why are long-lived plasma cells long-lived?Frontiers in immunology · 2026Review
- Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.Computational and structural biotechnology journal · 2026Article
- Intravital Microscopy of Osteocytes: A New Era in Bone Cell Biology.Current osteoporosis reports · 2025Review
- Bendable graded index lens-based laser-scanning optical-resolution photoacoustic microendoscopy.Optics letters · 2025Article
- Segmentation-free Radon transform algorithm to detect orientation and size of tissue structures in multiphoton microscopy images.Journal of biomedical optics · 2025Article
- FLIMB: fluorescence lifetime microendoscopy for metabolic and functional imaging of femoral marrow at subcellular resolution.Biomedical optics express · 2025Article
- Endothelial heterogeneity in bone marrow: insights across development, adult life and leukemia.Leukemia · 2025Review
- Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.iScience · 2024Article
- Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration.Nature communications · 2024Article
- Endothelial SMAD1/5 signaling couples angiogenesis to osteogenesis in juvenile bone.Communications biology · 2024Article
- Longitudinal intravital imaging of the bone marrow for analysis of the race for the surface in a murine osteomyelitis model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution.Nature communications · 2024Article
- Article
- A semi-automated cell tracking protocol for quantitative analyses of neutrophil swarming to sterile andbioRxiv : the preprint server for biology · 2023Article
- Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone.Nature protocols · 2023Review
- In good times and in bad: How plasma cells resolve stress for a life-long union with the bone marrow.Frontiers in immunology · 2023Article
- Visualizing vasculature and its response to therapy in the tumor microenvironment.Theranostics · 2023Review
- Intravital imaging to study cancer progression and metastasis.Nature reviews. Cancer · 2023Review
- Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bone marrow is a central organ of the immune system, which hosts complex interactions of bone and immune compartments critical for hematopoiesis, immunological memory, and bone regeneration. Although these processes take place over months, most existing imaging techniques allow us to follow snapshots of only a few hours, at subcellular resolution. Here, we develop a microendoscopic multi-photon imaging approach called LIMB (longitudinal intravital imaging of the bone marrow) to analyze cellular dynamics within the deep marrow. The approach consists of a biocompatible plate surgically fixated to the mouse femur containing a gradient refractive index lens. This microendoscope allows highly resolved imaging, repeatedly at the same regions within marrow tissue, over months. LIMB reveals extensive vascular plasticity during bone healing and steady-state homeostasis. To our knowledge, this vascular plasticity is unique among mammalian tissues, and we expect this insight will decisively change our understanding of essential phenomena occurring within the bone marrow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.