ArticleScientific reports2017
Enzymatic production of all fourteen partially acetylated chitosan tetramers using different chitin deacetylases acting in forward or reverse mode.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 78 citations in OpenAlex.
- Acetylation and accessibility ofCell surface (Amsterdam, Netherlands) · 2026Article
- Acid-catalysed rearrangement of acyl groups: synthesis of β-d-gluco aminocyclopentitols and carbanucleoside derivatives.RSC advances · 2025Article
- Nanomaterials modulate tumor-associated macrophages for the treatment of digestive system tumors.Bioactive materials · 2024Review
- Fast insights into chitosan-cleaving enzymes by simultaneous analysis of polymers and oligomers through size exclusion chromatography.Scientific reports · 2024Article
- Article
- Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities.Molecules (Basel, Switzerland) · 2023Review
- Cell wall composition inFrontiers in fungal biology · 2023Article
- Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities.Nature communications · 2022Article
- Simultaneous Determination of Glibenclamide and Silymarin Released from Chitosan Microparticles by HPLC-ESI-MS Technique: Method Development and Validation.Pharmaceutics · 2022Article
- Chitosan: Sources, Processing and Modification Techniques.Gels (Basel, Switzerland) · 2022Review
- Customized chitooligosaccharide production-controlling their lengthFrontiers in bioengineering and biotechnology · 2022Article
- Deciphering the ChitoCode: fungal chitins and chitosans as functional biopolymers.Fungal biology and biotechnology · 2021Article
- Article
- In silico and in vitro analysis of an Aspergillus niger chitin deacetylase to decipher its subsite sugar preferences.The Journal of biological chemistry · 2021Article
- Production of Structurally Defined Chito-Oligosaccharides with a SingleJournal of agricultural and food chemistry · 2021Article
- Crustacean Waste-Derived Chitosan: Antioxidant Properties and Future Perspective.Antioxidants (Basel, Switzerland) · 2021Review
- Systematic Structural Characterization of Chitooligosaccharides Enabled by Automated Glycan Assembly.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases.International journal of molecular sciences · 2020Review
- Conversion of Chitin to Defined Chitosan Oligomers: Current Status and Future Prospects.Marine drugs · 2019Review
- Chitin and Chitosans: Characteristics, Eco-Friendly Processes, and Applications in Cosmetic Science.Marine drugs · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some of the most abundant biomolecules on earth are the polysaccharides chitin and chitosan of which especially the oligomeric fractions have been extensively studied regarding their biological activities. However, most of these studies have not been able to assess the activity of a single, defined, partially acetylated chitosan oligosaccharide (paCOS). Instead, they have typically analyzed chemically produced, rather poorly characterized mixtures, at best with a single, defined degree of polymerization (DP) and a known average degree of acetylation (DA), as no pure and well-defined paCOS are currently available. We here present data on the enzymatic production of all 14 possible partially acetylated chitosan tetramers, out of which four were purified (>95%) regarding DP, DA, and pattern of acetylation (PA). We used bacterial, fungal, and viral chitin deacetylases (CDAs), either to partially deacetylate the chitin tetramer; or to partially re-N-acetylate the glucosamine tetramer. Both reactions proceeded with surprisingly strong and enzyme-specific regio-specificity. These pure and fully defined chitosans will allow to investigate the particular influence of DP, DA, and PA on the biological activities of chitosans, improving our basic understanding of their modes of action, e.g. their molecular perception by patter recognition receptors, but also increasing their usefulness in industrial applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.