ArticleStem cell research & therapy2017
Low-dose strontium stimulates osteogenesis but high-dose doses cause apoptosis in human adipose-derived stem cells via regulation of the ERK1/2 signaling pathway.
Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 132 citations in OpenAlex.
- Biomaterial Screening Identifies Enhanced Osteogenic and Angiogenic Potential of Mn-Doped Calcium Phosphate Coatings.ACS biomaterials science & engineering · 2026Article
- Subchondral bone repair potential of an osteochondral scaffold augmented with BMP-2 or strontium-enriched amorphous calcium phosphate: a co-cultureFrontiers in medicine · 2026Article
- SrO-Containing Bioactive Glasses in the SiOACS biomaterials science & engineering · 2025Review
- Fabrication of Lithium/Strontium-Releasing Smart Bioactive Glasses with Anti-Inflammatory and Osteogenic Effects Tailored to Pathological Stages.Macromolecular bioscience · 2025Article
- From hard tissues to beyond: Progress and challenges of strontium-containing biomaterials in regenerative medicine applications.Bioactive materials · 2025Review
- 3D-Printed Cell-Instructive Scaffolds Based onACS biomaterials science & engineering · 2025Article
- Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow.Journal of biological engineering · 2025Article
- Effects of 0.01 mM strontium on human periodontal ligament stem cell osteogenic differentiation via the Wnt/The Journal of international medical research · 2025Article
- Osteogenic effects of electrophoretically deposited Sr-doped calcium silicate coatings on titanium.Frontiers in bioengineering and biotechnology · 2025Article
- Harnessing strontium in regenerative dentistry: progress and perspectives.Frontiers in bioengineering and biotechnology · 2025Review
- Strontium-doped hydroxyapatite microspheres loaded with iloprost promote dentin-pulp complex regeneration.Frontiers in bioengineering and biotechnology · 2025Article
- Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024Review
- Strontium and Zinc Co-Doped Mesoporous Bioactive Glass Nanoparticles for Potential Use in Bone Tissue Engineering Applications.Nanomaterials (Basel, Switzerland) · 2024Article
- Intrafibrillar mineralization of type I collagen with calcium carbonate and strontium carbonate induced by polyelectrolyte-cation complexes.Nanoscale advances · 2024Article
- A phase-transited lysozyme coating doped with strontium on titanium surface for bone repairing via enhanced osteogenesis and immunomodulatory.Frontiers in cell and developmental biology · 2024Article
- Biosynthesized Silver Nanoparticles fromCurrent pharmaceutical biotechnology · 2024Article
- Influence of Strontium on the Biological Behavior of Bioactive Glasses for Bone Regeneration.Materials (Basel, Switzerland) · 2023Review
- Association of Urinary Strontium with Cardiovascular Disease Among the US Adults: A Cross-Sectional Analysis of the National Health and Nutrition Examination Survey.Biological trace element research · 2023Article
- The Role of Trace Elements and Minerals in Osteoporosis: A Review of Epidemiological and Laboratory Findings.Biomolecules · 2023Review
- How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundStrontium is a widely used anti-osteoporotic agent due to its dual effects on inhibiting bone resorption and stimulating bone formation. Thus, we studied the dose response of strontium on osteo-inductive efficiency in human adipose-derived stem cells (hASCs).
methodQualitative alkaline phosphatase (ALP) staining, quantitative ALP activity, Alizarin Red staining, real-time polymerase chain reaction and Western blot were used to investigate the in vitro effects of a range of strontium concentrations on hASC osteogenesis and associated signaling pathways.
resultsIn vitro work revealed that strontium (25-500 μM) promoted osteogenic differentiation of hASCs according to ALP activity, extracellular calcium deposition, and expression of osteogenic genes such as runt-related transcription factor 2, ALP, collagen-1, and osteocalcin. However, osteogenic differentiation of hASCs was significantly inhibited with higher doses of strontium (1000-3000 μM). These latter doses of strontium promoted apoptosis, and phosphorylation of ERK1/2 signaling was increased and accompanied by the downregulation of Bcl-2 and increased phosphorylation of BAX. The inhibition of ERK1/2 decreased apoptosis in hASCs.
conclusionLower concentrations of strontium facilitate osteogenic differentiation of hASCs up to a point; higher doses cause apoptosis of hASCs, with activation of the ERK1/2 signaling pathway contributing to this process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.