Evidence map›Paper›PMID 29253503›Full record

ArticleJournal of immunological methods2018

FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F.

Wendy E Geslewitz, Caroline M Percopo, Helene F Rosenberg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Eosinophils in chronic obstructive pulmonary disease.Therapeutic advances in respiratory disease
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Wendy E GeslewitzInflammation Immunobiology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Caroline M PercopoInflammation Immunobiology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Helene F RosenbergInflammation Immunobiology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Electronic address: hrosenberg@niaid.nih.gov.
National Institutes of Health · US

Funding

Eosinophils, Inflammation and ImmunityZIAAI000941 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI ROSENBERG, HELENE · 2009 to 2020
$8.0M
Biology of the Eosinophilic LeukocyteZ01AI000941 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI ROSENBERG, HELENE · 2004 to 2008
$1.6M
Intramural NIH HHS Z01 AI000941Intramural NIH HHS ZIA AI000941
6 · The paper itself

Abstract

Flow cytometry protocols designed to identify mouse eosinophils typically target Siglec F, an α-2,3-sialic acid binding transmembrane protein expressed universally on cells of this lineage. While a convenient target, antibody-mediated ligation of Siglec F induces eosinophil apoptosis, which limits its usefulness for isolations that are to be followed by functional and/or gene expression studies. We present here a method for FACS isolation which does not target Siglec F and likewise utilizes no antibodies targeting IL5Rα (CD125) or CCR3. Single cell suspensions are prepared from lungs of mice that were sensitized and challenged with Aspergillus fumigatus antigens; eosinophils were identified and isolated by FACS as live SSC

Indexed as

AnimalsAntibodiesAntigens, Differentiation, MyelomonocyticEosinophilsFlow CytometryInterleukin-5 Receptor alpha SubunitLungMiceMice, Inbred BALB CMice, TransgenicReceptors, CCR3Sialic Acid Binding Immunoglobulin-like LectinsAntibodiesAntigens, Differentiation, MyelomonocyticCcr3 protein, mouseInterleukin-5 Receptor alpha SubunitReceptors, CCR3Sialic Acid Binding Immunoglobulin-like LectinsSiglecf protein, mouseAllergenInflammationInterleukin-5

Identifiers

PMID29253503
PMCPMC5818306
OpenAlexW2772577425

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.