ArticleJournal of experimental botany2018
The GhmiR157a-GhSPL10 regulatory module controls initial cellular dedifferentiation and callus proliferation in cotton by modulating ethylene-mediated flavonoid biosynthesis.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 82 citations in OpenAlex.
- Single-cell RNA sequencing reveals developmental trajectories and environmental regulation of callus formation in Arabidopsis.Stress biology · 2025Article
- Analysis of transcriptome and differential expression of different types of calli of Eucalyptus urophylla × Eucalyptus grandis.PloS one · 2025Article
- Integrated analysis of microRNAs and lncRNAs expression profiles reveals regulatory modules during adventitious shoot induction in Moringa oleifera Lam.BMC plant biology · 2024Article
- miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways.International journal of molecular sciences · 2024Article
- Transcriptional regulation of flavonol biosynthesis in plants.Horticulture research · 2024Review
- Integrated metabolomics and transcriptomics analysis highlight key pathways involved in the somatic embryogenesis of Darjeeling tea.BMC genomics · 2024Article
- The miR156/SPL12 module orchestrates fruit colour change through directly regulating ethylene production pathway in blueberry.Plant biotechnology journal · 2024Article
- Riboflavin mediates m6A modification targeted by miR408, promoting early somatic embryogenesis in longan.Plant physiology · 2023Article
- Light-Dependent Regulatory Interactions between the Redox System and miRNAs and Their Biochemical and Physiological Effects in Plants.International journal of molecular sciences · 2023Review
- GWAS across multiple environments and WGCNA suggest the involvement of ZmARF23 in embryonic callus induction from immature maize embryos.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Regulatory roles of noncoding RNAs in callus induction and plant cell dedifferentiation.Plant cell reports · 2023Review
- Uniconazole Augments Abscisic Acid in Promoting Somatic Embryogenesis in Cotton (Frontiers in plant science · 2022Article
- Genome-wide Characterization of the JmjC Domain-Containing Histone Demethylase Gene Family Reveals GhJMJ24 and GhJMJ49 Involving in Somatic Embryogenesis Process in Cotton.Frontiers in molecular biosciences · 2022Article
- Computational screening of miRNAs and their targets in saffron (Crocus sativus L.) by transcriptome mining.Planta · 2021Article
- Comparative transcriptome analysis uncovers cell wall reorganization and repressed cell division during cotton fiber initiation.BMC developmental biology · 2021Article
- miRNAs as key regulators via targeting the phytohormone signaling pathways during somatic embryogenesis of plants.3 Biotech · 2020Review
- Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants.International journal of molecular sciences · 2020Review
- Transcriptional reprogramming strategies and miRNA-mediated regulation networks of Taxus media induced into callus cells from tissues.BMC genomics · 2020Article
- MicroRNAs and their targeted genes associated with phase changes of stem explants during tissue culture of tea plant.Scientific reports · 2019Article
- Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton.International journal of molecular sciences · 2019Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
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Abstract
MicroRNAs (miRNAs) modulate many biological processes through inactivation of specific mRNA targets such as those encoding transcription factors. A delicate spatial/temporal balance between specific miRNAs and their targets is central to achieving the appropriate biological outcomes. Somatic embryogenesis in cotton (Gossypium hirsutum), which goes through initial cellular dedifferentiation, callus proliferation, and somatic embryo development, is of great importance for both fundamental research and biotechnological applications. In this study, we characterize the function of the GhmiR157a-GhSPL10 miRNA-transcription factor module during somatic embryogenesis in cotton. We show that overexpression of GhSPL10, a target of GhmiR157a, increases free auxin and ethylene content and expression of associated signaling pathways, activates the flavonoid biosynthesis pathway, and promotes initial cellular dedifferentiation and callus proliferation. Inhibition of expression of the flavonoid synthesis gene F3H in GhSPL10 overexpression lines (35S:rSPL10-7) blocked callus initiation, while exogenous application of several types of flavonol promoted callus proliferation, associated with cell cycle-related gene expression. Inhibition of ethylene synthesis by aminoethoxyvinylglycine treatment in the 35S:rSPL10-7 line severely inhibited callus initiation, while activation of ethylene signaling through 1-aminocyclopropane 1-carboxylic acid treatment, EIN2 overexpression, or inhibition of the ethylene negative regulator CTR1 by RNA interference promoted flavonoid-related gene expression and flavonol accumulation. These results show that an up-regulation of ethylene signaling and the activation of flavonoid biosynthesis in GhSPL10 overexpression lines were associated with initial cellular dedifferentiation and callus proliferation. Our results demonstrate the importance of a GhmiR157a-GhSPL10 gene module in regulating somatic embryogenesis via hormonal and flavonoid pathways.
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