ArticleNature biotechnology2018
Virus stamping for targeted single-cell infection in vitro and in vivo.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
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- CRISPR-clear imaging of melanin-rich B16-derived solid tumors.Communications biology · 2023Article
- Advances in optical molecular imaging for neural visualization.Frontiers in bioengineering and biotechnology · 2023Review
- All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain.Cell reports methods · 2022Article
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- AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset.Nature neuroscience · 2022Article
- Red Light Optogenetics in Neuroscience.Frontiers in cellular neuroscience · 2021Review
- BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System.iScience · 2020Article
- Near-Infrared Laser-Based Spatially Targeted Nano-enhanced Optical Delivery of Therapeutic Genes to Degenerated Retina.Molecular therapy. Methods & clinical development · 2020Article
- Kin discrimination in social yeast is mediated by cell surface receptors of the Flo11 adhesin family.eLife · 2020Article
- New Optical Tools to Study Neural Circuit Assembly in the Retina.Frontiers in neural circuits · 2020Review
- Magnetically guided virus stamping for the targeted infection of single cells or groups of cells.Nature protocols · 2019Article
- Next-generation interfaces for studying neural function.Nature biotechnology · 2019Review
- PatcherBot: a single-cell electrophysiology robot for adherent cells and brain slices.Journal of neural engineering · 2019Article
- The first steps in vision: cell types, circuits, and repair.EMBO molecular medicine · 2019Review
- Accurate signal-source localization in brain slices by means of high-density microelectrode arrays.Scientific reports · 2019Article
- Genetic Modification of Brain Organoids.Frontiers in cellular neuroscience · 2019Article
- Cell-type-specific and projection-specific brain-wide reconstruction of single neurons.Nature methods · 2018Article
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Authors and funding
20 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic engineering by viral infection of single cells is useful to study complex systems such as the brain. However, available methods for infecting single cells have drawbacks that limit their applications. Here we describe 'virus stamping', in which viruses are reversibly bound to a delivery vehicle-a functionalized glass pipette tip or magnetic nanoparticles in a pipette-that is brought into physical contact with the target cell on a surface or in tissue, using mechanical or magnetic forces. Different single cells in the same tissue can be infected with different viruses and an individual cell can be simultaneously infected with different viruses. We use rabies, lenti, herpes simplex, and adeno-associated viruses to drive expression of fluorescent markers or a calcium indicator in target cells in cell culture, mouse retina, human brain organoid, and the brains of live mice. Virus stamping provides a versatile solution for targeted single-cell infection of diverse cell types, both in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.