ArticleParasite (Paris, France)2017
Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting.
Article in Parasite (Paris, France), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Loop-Mediated Isothermal Amplification (LAMP) Assessment for Malaria Diagnosis in Equatorial Guinea.Pathogens (Basel, Switzerland) · 2026Article
- Implementation of a Composite Reference Standard Methodology in the Greek National Malaria Reference Center, Insights from a Seven-Year Experience.Pathogens (Basel, Switzerland) · 2026Article
- Five Hot Topics in Tropical Medicine: 2025.Infectious disease reports · 2026Review
- A comparison of the cost-effectiveness of the current standard of care and of the haematology analyser XN31-microscopy combination for diagnosing malaria in a nonendemic country.Memorias do Instituto Oswaldo Cruz · 2026Article
- Malaria diagnosis using a combined system of a simple and fast extraction method with a lyophilised Dual-LAMP assay in a non-endemic setting.Pathogens and global health · 2024Article
- Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns.Applied microbiology and biotechnology · 2023Article
- A Diagnostic Accuracy Study to Evaluate Standard Rapid Diagnostic Test (RDT) Alone to Safely Rule Out Imported Malaria in Children Presenting to UK Emergency Departments.Journal of the Pediatric Infectious Diseases Society · 2023Article
- MINI: A high-throughput point-of-care device for performing hundreds of nucleic acid tests per day.Biosensors & bioelectronics · 2022Article
- Application of a low-cost, specific, and sensitive loop-mediated isothermal amplification (LAMP) assay to detect Plasmodium falciparum imported from Africa.Molecular and biochemical parasitology · 2022Article
- Usefulness of a commercial LAMP assay for detection of malaria infection, including Plasmodium knowlesi cases, in returning travelers in Spain.BMC research notes · 2022Article
- Fast detection and quantification of Plasmodium species infected erythrocytes in a non-endemic region by using the Sysmex XN-31 analyzer.Malaria journal · 2022Article
- Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting.Parasite (Paris, France) · 2022Article
- Malaria diagnosis in a malaria non-endemic high-resource country: high variation of diagnostic strategy in clinical laboratories in the Netherlands.Malaria journal · 2021Article
- Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.Diagnostics (Basel, Switzerland) · 2021Review
- Loop-mediated isothermal amplification (LAMP) assay for the diagnosis of imported malaria: a narrative review.Le infezioni in medicina · 2021Review
- Diagnostic Methods for Non-Falciparum Malaria.Frontiers in cellular and infection microbiology · 2021Review
- Rapid diagnostic tests failing to detect infections by Plasmodium falciparum encoding pfhrp2 and pfhrp3 genes in a non-endemic setting.Malaria journal · 2020Article
- Case Report: Hyperreactive Malarial Splenomegaly Syndrome Diagnosed with Loop-Mediated Isothermal Amplification and Treated with Artemisinin-Based Combination Therapy.The American journal of tropical medicine and hygiene · 2019Article
- Use of Loop-Mediated Isothermal Amplification in a Resource-Saving Strategy for Primary Malaria Screening in a Non-Endemic Setting.The American journal of tropical medicine and hygiene · 2019Article
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSensitive and easy-to-perform methods for the diagnosis of malaria are not yet available. Improving the limit of detection and following the requirements for certification are issues to be addressed in both endemic and non-endemic settings. The aim of this study was to test whether loop-mediated isothermal amplification of DNA (LAMP) may be an alternative to microscopy or real-time PCR for the screening of imported malaria cases in non-endemic area.
results310 blood samples associated with 829 suspected cases of imported malaria were tested during a one year period. Microscopy (thin and thick stained blood slides, reference standard) was used for the diagnosis. Real-time PCR was used as a standard of truth, and LAMP (Meridian Malaria Plus) was used as an index test in a prospective study conducted following the Standards for Reporting Diagnosis Accuracy Studies. In the 83 positive samples, species identification was P. falciparum (n = 66), P. ovale (n = 9), P. vivax (n = 3) P. malariae (n = 3) and 2 co-infections with P. falciparum + P.malariae. Using LAMP methods, 93 samples gave positive results, including 4 false-positives. Sensitivity, specificity, positive predictive value and negative predictive value for LAMP tests were 100%, 98.13%, 95.51%, and 100% compared to PCR.
conclusionHigh negative predictive value, and limit of detection suggest that LAMP can be used for screening of imported malaria cases in non-endemic countries when expert microscopists are not immediately available. However, the rare occurrence of non-valid results and the need for species identification and quantification of positive samples preclude the use of LAMP as a single reference method.
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