ArticlePlant biotechnology journal2018
Precise editing of CLAVATA genes in Brassica napus L. regulates multilocular silique development.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Advanced breeding techniques inPlant signaling & behavior · 2026Review
- Efficient CRISPR/Cas-SF01 genome editing tools with high editing efficiency in allotetraploid oilseed rape.Journal of integrative plant biology · 2026Article
- Precise Creation of Elite Multilocular Germplasm Using a CBE NG System in Brassica napus.Plant biotechnology journal · 2026Article
- At the tip: novel regulators of shoot apical meristem development in canola.Plant physiology · 2026Article
- Natural allelic variation in BnCLV1 orchestrates root architectural remodelling and yield performance under nitrogen-limited conditions in Brassica napu.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- CRISPR/Cas9-mediated mutagenesis ofFrontiers in plant science · 2026Article
- Marker Metabolite-Based Multi-Omics Analysis Identifies New Loci Controlling Thousand Seed Weight in Brassica Napus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Large-scale screening of genes responsible for silique length and seed size in Brassica Napus via pooled CRISPR library.BMC genomics · 2025Article
- A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus.Plant biotechnology journal · 2025Article
- Efficient and versatile rapeseed transformation for new breeding technologies.The Plant journal : for cell and molecular biology · 2025Article
- Review
- Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments.Functional & integrative genomics · 2025Review
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- Review
- Studying Salt-Induced Shifts in Gene Expression Patterns of Glucosinolate Transporters and Glucosinolate Accumulation in Two ContrastingMetabolites · 2024Article
- Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate.GM crops & food · 2023Review
- Article
- Asymmetric Evolution of Protein Domains in the Leucine-Rich Repeat Receptor-Like Kinase Family of Plant Signaling Proteins.Molecular biology and evolution · 2023Article
- The current scenario and future perspectives of transgenic oilseed mustard by CRISPR-Cas9.Molecular biology reports · 2023Review
11 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multilocular silique is a desirable agricultural trait with great potential for the development of high-yield varieties of Brassica. To date, no spontaneous or induced multilocular mutants have been reported in Brassica napus, which likely reflects its allotetraploid nature and the extremely low probability of the simultaneous random mutagenesis of multiple gene copies with functional redundancy. Here, we present evidence for the efficient knockout of rapeseed homologues of CLAVATA3 (CLV3) for a secreted peptide and its related receptors CLV1 and CLV2 in the CLV signalling pathway using the CRISPR/Cas9 system and achieved stable transmission of the mutations across three generations. Each BnCLV gene has two copies located in two subgenomes. The multilocular phenotype can be recovered only in knockout mutations of both copies of each BnCLV gene, illustrating that the simultaneous alteration of multiple gene copies by CRISPR/Cas9 mutagenesis has great potential in generating agronomically important mutations in rapeseed. The mutagenesis efficiency varied widely from 0% to 48.65% in T
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