Evidence map›Paper›PMID 29250754›Full record

ArticleApplied biochemistry and biotechnology2018

High-Level Production of DNA-Specific Endonuclease AsEndI with Synonymous Codon and its Potential Utilization for Removing DNA Contamination.

Yuan Wang, Peng Jia, Rahat Sharif, Zhengchen Li, Yuhong Li, Peng Chen

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yuan WangCollege of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China.
Peng JiaCollege of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China.
Rahat SharifCollege of Horticulture, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China.
Zhengchen LiCollege of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China.
Yuhong LiCollege of Horticulture, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China. liyuhong73@nwsuaf.edu.cn.
Peng ChenCollege of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China. pengchen@nwsuaf.edu.cn.
Northwest A&F University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endonuclease I is a widely distributed periplasmic or extracellular enzyme. A method for the high-level production of recombinant AsEndI (endonuclease I from Aliivibrio salmonicida) in Escherichia coli with secretion expression is investigated. The coding sequence of AsEndI gene was assembled according to the E. coli codon usage bias, and AsEndI was expressed in the periplasm of E. coli TOP10 with a C-terminal 6× His-tagged fusion. The recombinant AsEndI (His-AsEndI) was purified by Ni-NTA resin with a yield of 1.29 × 10

Indexed as

CodonAliivibrio salmonicidaCulture MediaDeoxyribonuclease IDNA, Single-StrandedElectrophoresis, Polyacrylamide GelEscherichia coliHot TemperatureHydrogen-Ion ConcentrationPolymerase Chain ReactionRecombinant ProteinsRNASodium ChlorideCodonCulture MediaDeoxyribonuclease IDNA, Single-StrandedRecombinant ProteinsRNASodium ChlorideCodon optimizationDNA contaminationHigh saltNuclease activityPurification

Identifiers

PMID29250754
OpenAlexW2774271323

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.