ArticleJournal of endocrinological investigation2018
Molecular study of human sperm RNA: Ropporin and CABYR in asthenozoospermia.
Article in Journal of endocrinological investigation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Silent cells? Potential for context-dependent gene expression in mature sperm.Proceedings. Biological sciences · 2025Review
- Structure and Composition of Spermatozoa Fibrous Sheath in Diverse Groups of Metazoa.International journal of molecular sciences · 2024Review
- Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis.International journal of molecular sciences · 2023Article
- Production and Immunological Characterization of scFv Specific to Epitope ofTropical medicine and infectious disease · 2023Article
- Influence of Risk Factors for Male Infertility on Sperm Protein Composition.International journal of molecular sciences · 2021Review
- COVID-19-associated inhibition of energy accumulation pathways in human semen samples.F&S science · 2021Observational
- Advances in sperm analysis: techniques, discoveries and applications.Nature reviews. Urology · 2021Review
- Seminal exosomes - An important biological marker for various disorders and syndrome in human reproduction.Saudi journal of biological sciences · 2021Review
- Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.Frontiers in cell and developmental biology · 2021Article
- Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Frontiers in cell and developmental biology · 2021Article
- Screening, identification and interaction analysis of key MicroRNAs and genes in Asthenozoospermia.International journal of medical sciences · 2021Article
- Article
- Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.Nature communications · 2019Article
- In Silico Analysis of Sperm From Ejaculates with Different Semen Characteristics.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSperm motility is an essential aspect of human fertility. Sperm contain an abundance of transcripts, thought to be remnants of mRNA, which comprise a genetic fingerprint and can be considered a historic record of gene expression during spermatogenesis. The aberrant expression of numerous genes has been found to contribute to impaired sperm motility; these include ROPN1 (rhophilin associated tail protein 1), which encodes a component of the fibrous sheath of the mammalian sperm flagella, and CABYR (calcium-binding tyrosine-(Y)-phosphorylation-regulated protein), which plays an important role in calcium activation and modulation. The aim of this study was to investigate ROPN1 and CABYR gene co-expression in asthenozoospermic semen samples in comparison with normozoospermic samples.
methodsWe studied 120 semen samples (60 normozoospermic and 60 asthenozoospermic) from Caucasian patients attending our centre for an andrological check-up. Total RNA was extracted from purified spermatozoa with RNeasy mini kit. ROPN1 and CABYR mRNA expression was analysed using RT-qPCR. Continuous variables were described as means ± standard deviations.
resultsROPN1 and CABYR mRNA were simultaneously downregulated in asthenozoospermic in comparison with normozoospermic samples. There was also a positive correlation between total progressive motility and ROPN1 and CABYR gene expression and between total motile sperm number and ROPN1 and CABYR gene expression.
conclusionsThe results demonstrated downregulation of both ROPN1 and CABYR in asthenozoospermic samples and importantly, a positive correlation between the expression of the two genes, suggesting that ROPN1 and CABYR co-expression is a prerequisite for normal flagellar function and sperm motility.
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