ArticleTransgenic research2018
Alteration of flower colour in Ipomoea nil through CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase 4.
Article in Transgenic research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Ectopic expression of the flavonoid 3',5'-hydroxylase gene in Ipomoea nil induces the accumulation of delphinidin-based anthocyanins in the petals and inhibits their opening.BMC plant biology · 2026Article
- Engineering plant architecture in the ornamental species Eustoma grandiflorum by knockout of strigolactone biosynthesis.Plant cell reports · 2026Article
- Molecular Control of Flower Colour Change in Angiosperms.Plants (Basel, Switzerland) · 2025Review
- Advancing ornamental plant breeding through genomic technologies: opportunities, challenges, and future directions.Functional & integrative genomics · 2025Review
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Combined enhancement of ascorbic acid, β-carotene and zeaxanthin in gene-edited lettuce.Plant biotechnology journal · 2025Article
- Review
- Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce.International journal of molecular sciences · 2025Article
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- In Vitro Culture Technology and Advanced Biotechnology Tools Toward Improvement in Gladiolus (Gladiolus species): Present Scenario and Future Prospects.Molecular biotechnology · 2024Review
- Flower color modification in Torenia fournieri by genetic engineering of betacyanin pigments.BMC plant biology · 2024Article
- Article
- Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update.Plants (Basel, Switzerland) · 2023Review
- Insights into Metabolic Engineering of Bioactive Molecules inCurrent genomics · 2023Review
- Phylogeny ofInternational journal of molecular sciences · 2023Article
- Insights into the Genes Involved in ABA Biosynthesis and Perception during Development and Ripening of the Chilean Strawberry Fruit.International journal of molecular sciences · 2023Article
- Biotechnologically Engineered Plants.Biology · 2023Review
- Metabolic engineering of plant secondary metabolites: prospects and its technological challenges.Frontiers in plant science · 2023Review
- CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops.Molecular biotechnology · 2022Review
- Genome-Wide Identification ofGenes · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Japanese morning glory, Ipomoea nil, exhibits a variety of flower colours, except yellow, reflecting the accumulation of only trace amounts of carotenoids in the petals. In a previous study, we attributed this effect to the low expression levels of carotenogenic genes in the petals, but there may be other contributing factors. In the present study, we investigated the possible involvement of carotenoid cleavage dioxygenase (CCD), which cleaves specific double bonds of the polyene chains of carotenoids, in the regulation of carotenoid accumulation in the petals of I. nil. Using bioinformatics analysis, seven InCCD genes were identified in the I. nil genome. Sequencing and expression analyses indicated potential involvement of InCCD4 in carotenoid degradation in the petals. Successful knockout of InCCD4 using the CRISPR/Cas9 system in the white-flowered cultivar I. nil cv. AK77 caused the white petals to turn pale yellow. The total amount of carotenoids in the petals of ccd4 plants was increased 20-fold relative to non-transgenic plants. This result indicates that in the petals of I. nil, not only low carotenogenic gene expression but also carotenoid degradation leads to extremely low levels of carotenoids.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.