Evidence map›Paper›PMID 29247317›Full record

ArticleMolecular biotechnology2018

Bilayer Amniotic Membrane/Nano-fibrous Fibroin Scaffold Promotes Differentiation Capability of Menstrual Blood Stem Cells into Keratinocyte-Like Cells.

Maryam Fard, Maryam Akhavan-Tavakoli, Sayeh Khanjani, Sona Zare, Haleh Edalatkhah, Shaghayegh Arasteh, Davood Mehrabani, Amir-Hassan Zarnani, Somaieh Kazemnejad, Reza Shirazi

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  14. A Review on Modifications of Amniotic Membrane for Biomedical Applications.Frontiers in bioengineering and biotechnology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Maryam FardDepartment of Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
Maryam Akhavan-TavakoliDepartment of Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
Sayeh KhanjaniReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran.
Sona ZareSkin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Haleh EdalatkhahReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran.
Shaghayegh ArastehReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran.
Davood MehrabaniStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran.
Amir-Hassan ZarnaniReproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Somaieh KazemnejadReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, P.O. Box: 1177-19615, Tehran, Iran. s.kazemnejad@avicenna.ac.ir.
Reza ShiraziDepartment of Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. shirazi.r@iums.ac.ir.ORCID http://orcid.org/0000-0002-3907-079X

Funding

Iran National Science Foundation (INSF) 91059597
6 · The paper itself

Abstract

The skin provides a dynamic barrier separating and protecting human body from the exterior world, and then immediate repair and rebuilding of the epidermal barrier is crucial after wound and injury. Wound healing without scars and complete regeneration of skin tissue still remain as a clinical challenge. The demand to engineer scaffolds that actively promote regeneration of damaged areas of the skin has been increased. In this study, menstrual blood-derived stem cells (MenSCs) have been induced to differentiate into keratinocytes-like cells in the presence of human foreskin-derived keratinocytes on a bilayer scaffold based on amniotic membrane and silk fibroin. Based on the findings, newly differentiated keratinocytes from MenSCs successfully expressed the keratinocytes specific markers at both mRNA and protein levels judged by real-time PCR and immunostaining techniques, respectively. We could show that the differentiated cells over bilayer composite scaffolds express the keratinocytes specific markers at higher levels when compared with those cultured in conventional 2D culture system. Based on these findings, bilayer amniotic membrane/nano-fibrous fibroin scaffold represents an efficient natural construct with broad applicability to generate keratinocytes from MenSCs for stem cell-based skin wounds healing and regeneration.

Indexed as

Tissue ScaffoldsAdultAmnionBiomarkersCell DifferentiationCoculture TechniquesFemaleFibroinsForeskinGene ExpressionHumansKeratin-14KeratinocytesMaleMembrane ProteinsMenstruationBiomarkersCKAP4 protein, humanFibroinsinvolucrinKeratin-14KRT14 protein, humanMembrane ProteinsProtein PrecursorsAmniotic membraneFibroinKeratinocytesMenstrual bloodScaffoldSkin woundStem cells

Identifiers

PMID29247317

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.