ArticleScientific reports2017
Host species adaptation of TLR5 signalling and flagellin recognition.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
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- Optimising the induction of inflammation within preterm infant-derived intestinal epithelial organoids.Npj gut and liver · 2026Article
- Therapeutic potential of Bacillus sonorensis PMC204 membrane vesicles against drug-resistant Mycobacterium tuberculosis.Medical microbiology and immunology · 2025Article
- Unveiling the multifaceted role of toll-like receptors in immunity of aquatic animals: pioneering strategies for disease management.Frontiers in immunology · 2024Review
- Gut microbiota and microbiota-derived metabolites in colorectal cancer: enemy or friend.World journal of microbiology & biotechnology · 2023Review
- Genome-Wide Identification and Characterization of Toll-like Receptors (TLRs) inJournal of fungi (Basel, Switzerland) · 2022Article
- Polysaccharides fromFrontiers in cellular and infection microbiology · 2022Article
- Immune defenses of the mammary gland epithelium of dairy ruminants.Frontiers in immunology · 2022Review
- Host and Species-Specificities of Pattern Recognition Receptors Upon Infection WithFrontiers in cellular and infection microbiology · 2022Review
- Probiotics and probiotic-based vaccines: A novel approach for improving vaccine efficacy.Frontiers in medicine · 2022Review
- NLRC4 Inflammasome-Mediated Regulation of Eosinophilic Functions.Immune network · 2021Article
- TLR-Mediated Signal Transduction and Neurodegenerative Disorders.Brain sciences · 2021Review
- Article
- Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality.Frontiers in immunology · 2021Article
- Inflammasome Activation in Bovine Peripheral Blood-Derived Macrophages Is Associated with Actin Rearrangement.Animals : an open access journal from MDPI · 2020Article
- Surface components and metabolites of probiotics for regulation of intestinal epithelial barrier.Microbial cell factories · 2020Review
- Escape of TLR5 Recognition byFrontiers in immunology · 2020Article
- The Toll Pathway in the Central Nervous System of Flies and Mammals.Neuromolecular medicine · 2018Review
- Functional expression of TLR5 of different vertebrate species and diversification in intestinal pathogen recognition.Scientific reports · 2018Article
- Toll-Like Receptors, Associated Biological Roles, and Signaling Networks in Non-Mammals.Frontiers in immunology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Toll-like receptor 5 (TLR5) recognition of flagellin instigates inflammatory signalling. Significant sequence variation in TLR5 exists between animal species but its impact on activity is less well understood. Building on our previous research that bovine TLR5 (bTLR5) is functional, we compared human and bovine TLR5 activity and signalling in cognate cell lines. bTLR5 induced higher levels of CXCL8 when expressed in bovine cells and reciprocal results were found for human TLR5 (hTLR5) in human cells, indicative of host cell specificity in this response. Analysis of Toll/interleukin-1 receptor (TIR) sequences indicated that these differential responses involve cognate MyD88 recognition. siRNA knockdowns and inhibitor experiments demonstrated that there are some host differences in signalling. Although, PI3K activation is required for bTLR5 signalling, mutating bTLR5 F798 to hTLR5 Y798 within a putative PI3K motif resulted in a significantly reduced response. All ruminants have F798 in contrast to most other species, suggesting that TLR5 signalling has evolved differently in ruminants. Evolutionary divergence between bovine and human TLR5 was also apparent in relation to responses measured to diverse bacterial flagellins. Our results underscore the importance of species specific studies and how differences may alter efficacy of TLR-based vaccine adjuvants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.