Evidence map›Paper›PMID 29247006›Full record

ArticleThe Journal of biological chemistry2018

A novel fluorescence assay for measuring phosphatidylserine decarboxylase catalysis.

Jae-Yeon Choi, Yulia V Surovtseva, Sam M Van Sickle, Jan Kumpf, Uwe H F Bunz, Choukri Ben Mamoun, Dennis R Voelker

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Jae-Yeon ChoiFrom the Basic Science Section, Department of Medicine, National Jewish Health, Denver, Colorado 80206.
Yulia V Surovtsevathe Yale Center for Molecular Discovery, West Haven, Connecticut 06516.
Sam M Van SickleFrom the Basic Science Section, Department of Medicine, National Jewish Health, Denver, Colorado 80206.
Jan Kumpfthe Organisch-Chemisches Institut, Ruprecht-Karls-Universitat, Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Uwe H F Bunzthe Organisch-Chemisches Institut, Ruprecht-Karls-Universitat, Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Choukri Ben Mamounthe Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine, New Haven, Connecticut 06520, and.
Dennis R VoelkerFrom the Basic Science Section, Department of Medicine, National Jewish Health, Denver, Colorado 80206, voelkerd@njhealth.org.
University of Colorado Denver · USHeidelberg University · DEYale University · US

Funding

Function and Inhibition of Plasmodium Lipid DecarboxylasesR01AI097218 · NIAID · YALE UNIVERSITY · PI BEN MAMOUN, CHOUKRI · 2012 to 2016
$2.0M
Structure and Function of Eukaryotic Phosphatidylserine DecarboxylaseR01GM104485 · NIGMS · NATIONAL JEWISH HEALTH · PI VOELKER, DENNIS R. · 2013 to 2016
$1.2M
NIAID NIH HHS R01 AI097218NIGMS NIH HHS R01 GM104485
6 · The paper itself

Abstract

Phosphatidylserine decarboxylases (PSDs) are central enzymes in phospholipid metabolism that produce phosphatidylethanolamine (PE) in bacteria, protists, plants, and animals. We developed a fluorescence-based assay for selectively monitoring production of PE in reactions using a maltose-binding protein fusion with

Indexed as

FluorescenceCarboxy-LyasesMaltose-Binding ProteinsPhosphatidylethanolaminesPlasmodium knowlesiProtozoan ProteinsRecombinant Fusion ProteinsCarboxy-LyasesMaltose-Binding ProteinsphosphatidylethanolaminePhosphatidylethanolaminesphosphatidylserine decarboxylaseProtozoan ProteinsRecombinant Fusion Proteinsfluorescenceinhibitorinhibitor screeningmembrane biogenesisphosphatidylserine decarboxylasephospholipid

Identifiers

PMID29247006
PMCPMC5798280
OpenAlexW2771093031

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.