ReviewInternational journal of molecular sciences2017
Impact of Methods on the Measurement of mRNA Turnover.
Review in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed.
- TTDB: a comprehensive Transcriptome Turnover Database for exploring mRNA stability.Nucleic acids research · 2026Article
- The impact of ribosome association on lncRNA stability: a new layer of post-transcriptional control?Biochemical Society transactions · 2025Review
- Article
- Minimal Perturbation Analysis of mRNA Degradation Rates with Tet-Off and RT-qPCR.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Disrupted Post-Transcriptional Regulation of Gene Expression as a Hallmark of Fatty Liver Progression.International journal of molecular sciences · 2024Article
- Nuclear mRNA decay: regulatory networks that control gene expression.Nature reviews. Genetics · 2024Review
- Nuclear export is a limiting factor in eukaryotic mRNA metabolism.PLoS computational biology · 2024Article
- Coordination of rhythmic RNA synthesis and degradation orchestrates 24- and 12-h RNA expression patterns in mouse fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transcriptional Comparison of Human and Murine Retinal Neovascularization.Investigative ophthalmology & visual science · 2023Article
- Coordination of rhythmic RNA synthesis and degradation orchestrates 24-hour and 12-hour RNA expression patterns in mouse fibroblasts.bioRxiv : the preprint server for biology · 2023Article
- bakR: uncovering differential RNA synthesis and degradation kinetics transcriptome-wide with Bayesian hierarchical modeling.RNA (New York, N.Y.) · 2023Article
- Assessing the Role of Cold-Shock Protein C: a Novel Regulator of Acinetobacter baumannii Biofilm Formation and Virulence.Infection and immunity · 2022Article
- Pan-cancer analysis of mRNA stability for decoding tumour post-transcriptional programs.Communications biology · 2022Article
- Biochemical pathways of 8-aminoguanine production in Sprague-Dawley and Dahl salt-sensitive rats.Biochemical pharmacology · 2022Article
- Post-transcriptional regulation during stress.FEMS yeast research · 2022Review
- Transcription feedback dynamics in the wake of cytoplasmic mRNA degradation shutdown.Nucleic acids research · 2022Article
- Global SLAM-seq for accurate mRNA decay determination and identification of NMD targets.RNA (New York, N.Y.) · 2022Article
- StaRTrEK:in silico estimation of RNA half-lives from genome-wide time-course experiments without transcriptional inhibition.BMC bioinformatics · 2022Article
- Estimating RNA dynamics using one time point for one sample in a single-pulse metabolic labeling experiment.BMC bioinformatics · 2022Article
- Exosomes as Natural Nanocarriers for RNA-Based Therapy and Prophylaxis.Nanomaterials (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The turnover of the RNA molecules is determined by the rates of transcription and RNA degradation. Several methods have been developed to study RNA turnover since the beginnings of molecular biology. Here we summarize the main methods to measure RNA half-life: transcription inhibition, gene control, and metabolic labelling. These methods were used to detect the cellular activity of the mRNAs degradation machinery, including the exo-ribonuclease Xrn1 and the exosome. On the other hand, the study of the differential stability of mature RNAs has been hampered by the fact that different methods have often yielded inconsistent results. Recent advances in the systematic comparison of different method variants in yeast have permitted the identification of the least invasive methodologies that reflect half-lives the most faithfully, which is expected to open the way for a consistent quantitative analysis of the determinants of mRNA stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.