ArticleeLife2017
Diverse functions of homologous actin isoforms are defined by their nucleotide, rather than their amino acid sequence.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 65 citations in OpenAlex.
- Molecular genotype-phenotype correlation in ACTB- and ACTG1-related non-muscle actinopathies.American journal of human genetics · 2026Article
- Cerebral organoids expressing mutant actin genes reveal cellular mechanism underlying microcephaly.EMBO reports · 2026Article
- Multiscale characterization ofiScience · 2025Article
- The complete absence of cytoplasmic γ-actin results in no discernible phenotype in mice or primary fibroblasts.The FEBS journal · 2025Article
- Amino acid-level differences in alpha-tubulin sequences are uniquely required for meiosis.Molecular biology of the cell · 2025Article
- The divergent intron-containing actin in sponge morphogenetic processes.NAR genomics and bioinformatics · 2025Article
- Do actin isoforms have unique functionalities at the protein level?Nature communications · 2025Article
- Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.Biomolecules · 2025Review
- β-actin function in platelets and red blood cells can be performed by γ-actin and is therefore independent of actin isoform protein sequence.Molecular biology of the cell · 2025Article
- Filamin A regulates platelet shape change and contractile force generation via phosphorylation of the myosin light chain.The Biochemical journal · 2024Article
- IL-2 delivery to CD8Nature communications · 2024Article
- The DIAPH3 linker specifies a β-actin network that maintains RhoA and Myosin-II at the cytokinetic furrow.Nature communications · 2024Article
- Article
- RNAi-directed knockdown in the cnidarian fish blood parasite Sphaerospora molnari.Scientific reports · 2024Article
- Article
- Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function.Nature communications · 2023Article
- Cytochalasans and Their Impact on Actin Filament Remodeling.Biomolecules · 2023Review
- SPLASH: a statistical, reference-free genomic algorithm unifies biological discovery.bioRxiv : the preprint server for biology · 2023Article
- Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogseLife · 2023Article
- Actin polymerization and depolymerization in developing vertebrates.Frontiers in physiology · 2023Review
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9 authors at 2 institutions in 1 country.
Funding
Abstract
β- and γ-cytoplasmic actin are nearly indistinguishable in their amino acid sequence, but are encoded by different genes that play non-redundant biological roles. The key determinants that drive their functional distinction are unknown. Here, we tested the hypothesis that β- and γ-actin functions are defined by their nucleotide, rather than their amino acid sequence, using targeted editing of the mouse genome. Although previous studies have shown that disruption of β-actin gene critically impacts cell migration and mouse embryogenesis, we demonstrate here that generation of a mouse lacking β-actin protein by editing β-actin gene to encode γ-actin protein, and vice versa, does not affect cell migration and/or organism survival. Our data suggest that the essential in vivo function of β-actin is provided by the gene sequence independent of the encoded protein isoform. We propose that this regulation constitutes a global 'silent code' mechanism that controls the functional diversity of protein isoforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.