ArticleMicrobial biotechnology2018
Molecular viability testing of viable but non-culturable bacteria induced by antibiotic exposure.
Article in Microbial biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- A photoactivation-free viability PCR reagent for rapid, culture-independent detection of live bacteria.Microbiology spectrum · 2026Article
- Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives.Computational and structural biotechnology journal · 2025Review
- Clinical relevance of Pseudomonas aeruginosa viable but non-culturable forms in cystic fibrosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Legionella colonization and 3D spatial location within a Pseudomonas biofilm.Scientific reports · 2024Article
- Prolonged storage reduces viability ofFrontiers in microbiology · 2024Article
- Involvement of Acquired Tobramycin Resistance in the Shift to the Viable but Non-Culturable State inInternational journal of molecular sciences · 2023Article
- Recent Methods for the Viability Assessment of Bacterial Pathogens: Advances, Challenges, and Future Perspectives.Pathogens (Basel, Switzerland) · 2022Review
- Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment.Applied microbiology and biotechnology · 2022Article
- Real-time PCR has advantages over culture-based methods in identifying major airway bacterial pathogens in chronic obstructive pulmonary disease: Results from three clinical studies in Europe and North America.Frontiers in microbiology · 2022Article
- Steady-State Pre-rRNA Analysis to Investigate the Functional Microbiome.Current protocols · 2021Article
- Sputum sample positivity for Haemophilus influenzae or Moraxella catarrhalis in acute exacerbations of chronic obstructive pulmonary disease: evaluation of association with positivity at earlier stable disease timepoints.Respiratory research · 2021Observational
- Contribution of Drugs Interfering with Protein and Cell Wall Synthesis to the Persistence ofInternational journal of molecular sciences · 2021Article
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- A Quantitative Survey of Bacterial Persistence in the Presence of Antibiotics: Towards Antipersister Antimicrobial Discovery.Antibiotics (Basel, Switzerland) · 2020Article
- Role of Tobramycin in the Induction and Maintenance of Viable but Non-CulturableAntibiotics (Basel, Switzerland) · 2020Article
- Testing the Antimicrobial Characteristics of Wood Materials: A Review of Methods.Antibiotics (Basel, Switzerland) · 2020Review
- Transformation ofPathogens (Basel, Switzerland) · 2020Review
- Effect of Environmental Conditions on the Formation of the Viable but Nonculturable State ofFrontiers in microbiology · 2020Article
- A culture-dependent and metagenomic approach of household drinking water from the source to point of use in a developing country.Water research X · 2019Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid amplification-based methods are limited by their inability to discriminate between viable and dead cells. To overcome this drawback, propidium monoazide (PMA) combined with qPCR has been used to differentiate viable from nonviable cells in environmental samples. However, assessing bacterial physiology using PMA-qPCR remains a challenge due to its incapability of detecting metabolic activities, leading to overestimation of the viable bacteria population under an inactivation condition (e.g. antibiotic treatments). A recent advanced technique to amplify ribosomal RNA precursors (pre-rRNA) has been shown to detect viable cells because pre-rRNAs are intermediates in rRNA synthesis. This study investigated the effect of different types of antibiotics on the bacterial viability or viable but non-culturable (VBNC) state using both PMA-qPCR and pre-rRNA analyses with Pseudomonas aeruginosa. This study demonstrated that P. aeruginosa was more sensitive to colistin than it was to carbenicillin, gentamicin and levofloxacin. We could discriminate VBNCP. aeruginosa cells using PMA-qPCR when antibiotic pressure induced the VBNC state. Also, pre-rRNA was able to distinguish viable cells from colistin-inactivated bacteria cells, and it could detect the presence of VBNC and persister cells. Our results showed that these two molecular methods could successfully eliminate false-positive signals derived from antibiotics-inactivated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.